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PLIM-G4 SIGNED
Long-lived optical probes to image G-quadruplex DNA in live cells
Total Cost €
0EC-Contrib. €
0Partnership
0Views
0PLIM-G4 project word cloud
Explore the words cloud of the PLIM-G4 project. It provides you a very rough idea of what is the project "PLIM-G4" about.
live
intensity
concentration
automated
visualizing
track
molecule
microscopy
emission
proven
none
function
carry
fact
direct
switch
successfully
properly
date
biological
life
infancy
give
dna
mainly
tools
fixed
structures
deep
probe
roles
imaging
platinum
experimental
reported
selectivity
immuno
relatively
first
complexes
optical
successful
probes
rely
replication
time
small
image
lab
tetra
maintenance
there
host
transcription
interactions
suggesting
sequencing
dynamics
brightness
throughput
stranded
phosphorescence
play
staining
strategy
lifetime
synthesis
topologies
existence
fluoresce
quadruplexes
independent
affinity
limitations
lacking
interaction
telomere
phosphorescent
plim
cellular
cells
environment
Project "PLIM-G4" data sheet
The following table provides information about the project.
| Coordinator |
IMPERIAL COLLEGE OF SCIENCE TECHNOLOGY AND MEDICINE
Organization address contact info |
| Coordinator Country | United Kingdom [UK] |
| Total cost | 212˙933 € |
| EC max contribution | 212˙933 € (100%) |
| Programme |
1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility) |
| Code Call | H2020-MSCA-IF-2019 |
| Funding Scheme | MSCA-IF-EF-ST |
| Starting year | 2021 |
| Duration (year-month-day) | from 2021-02-01 to 2023-01-31 |
Partnership
Take a look of project's partnership.
| # | ||||
|---|---|---|---|---|
| 1 | IMPERIAL COLLEGE OF SCIENCE TECHNOLOGY AND MEDICINE | UK (LONDON) | coordinator | 212˙933.00 |
Map
Project objective
There has been increasing experimental evidence suggesting that tetra-stranded DNA structures (G-quadruplexes) play important biological roles in telomere function and maintenance, replication and transcription. The most direct evidence for their existence has come from immuno-staining in fixed cells as well as from recent deep sequencing studies. However, to date, we are still lacking tools that allows us to visualizing G-quadruplexes directly in live cells. While several small-molecule probes that fluoresce upon interaction with DNA have been reported, none of them have been successful at imaging G-quadruplexes in a cellular environment. This is mainly due to the fact that they rely on changes in intensity which are not possible to track properly in a cellular environment. Recently, the host lab reported a novel strategy to image G-quadruplexes in live cells. This approach makes use of the changes in emission lifetime (rather than intensity) of optical probes upon their interaction with different topologies of DNA. Since life-time is concentration independent, this approach can be successfully used to image G-quadruplexes in live cells. While this has proven to be a highly successful approach, it is still in its infancy since the probe developed so far has a number of limitations such as low brightness, relatively small lifetime range and low selectivity. Thus, this project aims to develop a new set of probes that address all these issues and use them to image the dynamics of G-quadruplexes in live cells in real time. I propose to develop platinum complexes (which 'switch-on' their phosphorescent upon interactions with DNA) with high affinity and selectivity for G-quadruplexes. To achieve this, novel approaches for automated synthesis and high-throughput analysis will be developed. The new probes will be used to carry out Phosphorescence Lifetime Imaging Microscopy (PLIM) studies to give evidence for the first time of the dynamics of G-quadruplexes in live cells.
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The information about "PLIM-G4" are provided by the European Opendata Portal: CORDIS opendata.
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lastchecktime (2024-07-27 5:09:34) correctly updated