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Molecular structure and cell cycle regulated assembly of the kinetochore

Total Cost €


EC-Contrib. €






 MolStruKT project word cloud

Explore the words cloud of the MolStruKT project. It provides you a very rough idea of what is the project "MolStruKT" about.

kinetochores    passenger    restraints    cenp    yeast    feedback    directing    mitosis    levels    subcomplexes    dynamic    understand    cross    characterizing    chromosomes    chemical    linking    centromeres    combined    kinetochore    insights    unveil    macromolecular    time    centromere    elucidate    mitotic    missing    eukaryotes    accurate    structure    architecture    ray    molecular    stable    subunit    functional    propagation    segregation    exit    phosphorylation    spectrometry    crystallography    complexes    attach    analyze    nucleosome    temporal    native    tumorigenesis    network    chromosomal    bind    soluble    replenishment    mechanistic    function    microtubules    proteins    fundamental    sensing    sister    spindle    thirdly    tension    significantly    stoichiometries    distance    protein    budding    fidelity    human    assembled    structures    systematic    chromatid    cxms    topological    identity    generations    mass    assembling    reveal    defects    first    confers    minichromosomes    tight    separation    aneuploidy    chromosome    secondly    assembly   

Project "MolStruKT" data sheet

The following table provides information about the project.


Organization address
postcode: 80539

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Project website
 Total cost 1˙499˙932 €
 EC max contribution 1˙499˙932 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2014-STG
 Funding Scheme ERC-STG
 Starting year 2015
 Duration (year-month-day) from 2015-06-01   to  2020-05-31


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 


 Project objective

Accurate chromosome segregation in eukaryotes requires the assembly of the macromolecular kinetochore complex at centromeres to attach chromosomes to the mitotic spindle. The kinetochore proteins are organized in stable subcomplexes that bind to dynamic microtubules and ensure fidelity of sister chromatid separation through feedback control. Characterizing the kinetochore structure will significantly advance our understanding of chromosome segregation and how defects in this process can lead to aneuploidy, which is associated with tumorigenesis. X-ray crystallography has provided detailed insights into the function of subcomplexes, however, a molecular analysis of the native kinetochore subunit architecture is still missing. I have recently combined chemical cross-linking with mass spectrometry (CXMS) which allows for the first time the topological analysis of native macromolecular protein structures by a comprehensive set of distance restraints. Applying this approach to kinetochores assembled on budding yeast minichromosomes I aim to elucidate the architecture of the native centromere-assembled kinetochore complex and analyze how tension sensing by the chromosomal passenger complex is integrated into the structure. Secondly, the systematic analyses of changes in phosphorylation levels and in protein stoichiometries of soluble and nucleosome-associated human kinetochore complexes will reveal how the tight temporal control of assembling a functional kinetochore in mitosis is achieved. Thirdly, I will investigate the architecture of the centromere-associated network of kinetochore proteins by CXMS to unveil its role in directing CENP-A replenishment at mitotic exit in order to maintain centromere identity through generations. The analysis of the native kinetochore structure in different functional states will provide fundamental mechanistic insights and help us to understand how this architecture confers fidelity to centromere propagation and chromosome segregation.


year authors and title journal last update
List of publications.
2019 Josef Fischböck-Halwachs, Sylvia Singh, Mia Potocnjak, Götz Hagemann, Victor Solis-Mezarino, Stephan Woike, Medini Ghodgaonkar-Steger, Florian Weissmann, Laura D Gallego, Julie Rojas, Jessica Andreani, Alwin Köhler, Franz Herzog
The COMA complex interacts with Cse4 and positions Sli15/Ipl1 at the budding yeast inner kinetochore
published pages: , ISSN: 2050-084X, DOI: 10.7554/elife.42879
eLife 8 2020-03-10
2017 Shyamal Mosalaganti, Jenny Keller, Anika Altenfeld, Michael Winzker, Pascaline Rombaut, Michael Saur, Arsen Petrovic, Annemarie Wehenkel, Sabine Wohlgemuth, Franziska Müller, Stefano Maffini, Tanja Bange, Franz Herzog, Herbert Waldmann, Stefan Raunser, Andrea Musacchio
Structure of the RZZ complex and molecular basis of its interaction with Spindly
published pages: 961-981, ISSN: 0021-9525, DOI: 10.1083/jcb.201611060
The Journal of Cell Biology 216/4 2019-04-18
2016 Alexander Heuck, Sonja Schitter-Sollner, Marcin Józef Suskiewicz, Robert Kurzbauer, Juliane Kley, Alexander Schleiffer, Pascaline Rombaut, Franz Herzog, Tim Clausen
Structural basis for the disaggregase activity and regulation of Hsp104
published pages: , ISSN: 2050-084X, DOI: 10.7554/eLife.21516
eLife 5 2019-04-18
2016 Seychelle M Vos, David Pöllmann, Livia Caizzi, Katharina B Hofmann, Pascaline Rombaut, Tomasz Zimniak, Franz Herzog, Patrick Cramer
Architecture and RNA binding of the human negative elongation factor
published pages: , ISSN: 2050-084X, DOI: 10.7554/eLife.14981
eLife 5 2019-04-18
2016 Elisabeth Schmidtmann, Tobias Anton, Pascaline Rombaut, Franz Herzog, Heinrich Leonhardt
Determination of local chromatin composition by CasID
published pages: 476-484, ISSN: 1949-1034, DOI: 10.1080/19491034.2016.1239000
Nucleus 7/5 2019-04-18
2017 Victor Solis-Mezarino, Franz Herzog
compleXView: a server for the interpretation of protein abundance and connectivity information to identify protein complexes
published pages: W276-W284, ISSN: 0305-1048, DOI: 10.1093/nar/gkx411
Nucleic Acids Research 45/W1 2019-04-18
2016 Herzog, Franz; Jenni, Simon; Rombaut, Pascaline; Musacchio, Andrea; Overlack, Katharina; van Gerwen, Suzan; Stege, Patricia; Vetter, Ingrid; Wohlgemuth, Sabine; Dimitrova, Yoana N.; Harrison, Stephen C.; John, Juliane; Petrovic, Arsen; Keller, Jenny; Liu, Yahui
Structure of the MIS12 Complex and Molecular Basis of Its Interaction with CENP-C at Human Kinetochores
published pages: 1028–1040.e15, ISSN: 0092-8674, DOI: 10.1016/j.cell.2016.10.005
Cell 167/4 2019-05-27
2016 John R. Weir, Alex C. Faesen, Kerstin Klare, Arsen Petrovic, Federica Basilico, Josef Fischböck, Satyakrishna Pentakota, Jenny Keller, Marion E. Pesenti, Dongqing Pan, Doro Vogt, Sabine Wohlgemuth, Franz Herzog, Andrea Musacchio
Insights from biochemical reconstitution into the architecture of human kinetochores
published pages: 249-253, ISSN: 0028-0836, DOI: 10.1038/nature19333
Nature 537/7619 2019-05-27
2016 Petrovic, Arsen; Musacchio, Andrea; Liu, Yahui; Keller, Jenny; Rombaut, Pascaline; Mosalaganti, Shyamal; Herzog, Franz; Raunser, Stefan
Insights from the reconstitution of the divergent outer kinetochore of Drosophila melanogaster
published pages: , ISSN: 2046-2441, DOI: 10.1098/rsob.150236
Open Biology 2 2019-05-27

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