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Lipid and Polarity SIGNED

The diffusion and nanoclustering of a polarity module in the lipid environment

Total Cost €

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EC-Contrib. €

0

Partnership

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 Lipid and Polarity project word cloud

Explore the words cloud of the Lipid and Polarity project. It provides you a very rough idea of what is the project "Lipid and Polarity" about.

mammalian    universal    forms    mutants    vesicle    ras    pole    activating    super    contribution    questions    yeast    components    though    quantification    influences    asymmetric    imaging    diffusion    shown    protein    cerevisiae    cells    signaling    dramatic    cellular    budding    central    pi4p    nanoclusters    trafficking    saccharomyces    dynamics    regulation    module    cdc42    family    organization    completely    maintenance    individual    internal    embryogenesis    gef    regulator    establishment    diseases    cap    phosphatidylserine    asymmetrically    entails    analyze    p2    eukaryotic    vivo    assembly    localization    tracking    biology    cdc24    small    psd2    pivotal    polar    molecules    interact    nano    polarity    single    clustering    cho1    ps    bem1    molecule    live    resolution    pe    scaffold    pm    plasma    play    powerful    imparts    i4    gtpase    leaflet    membranes    mechanisms    environment    lipids    cancer    demonstrated    proteins    function    lipid    psd1    localizes    rho    orientation    phospholipids    cell    spatial    membrane   

Project "Lipid and Polarity" data sheet

The following table provides information about the project.

Coordinator
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS 

Organization address
address: RUE MICHEL ANGE 3
city: PARIS
postcode: 75794
website: www.cnrs.fr

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country France [FR]
 Project website http://ibgc.cnrs.fr/
 Total cost 185˙076 €
 EC max contribution 185˙076 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2015
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2016
 Duration (year-month-day) from 2016-04-01   to  2018-03-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS FR (PARIS) coordinator 185˙076.00

Map

 Project objective

Cellular polarity is a universal feature of eukaryotic biology. Failure to control cell orientation has dramatic consequences on embryogenesis and diseases including cancer. The establishment of cellular polarity often entails the asymmetric distribution of plasma membrane (PM) lipids signaling proteins and vesicle trafficking. The function of Rho proteins in cell polarity has been demonstrated from mammalian cells to yeast. The small GTPase Cdc42 is a pivotal regulator of polarity establishment and maintenance. A central feature of Cdc42 function is its ability to interact with membrane. In budding yeast, Cdc42 localizes asymmetrically at the PM, at the pole of the cell, along with the scaffold protein Bem1 and the Cdc42 activating GEF Cdc24. The asymmetric distribution of lipids in the internal leaflet of the membrane play a crucial role in the regulation of Cdc42 activity and localization, though the mechanisms are not completely understood. The organization of lipids in the PM influences the spatial distribution and activity of key signaling proteins as Ras family proteins. Ras forms nanoclusters in cell membranes that are essential for signaling and Phosphatidylserine lipids have been shown to play a role in the assembly of K-Ras nanoclusters in mammalian cells. In this work, we will study Cdc42, Cdc24 and Bem1 dynamics in the membrane and particularly at the polar cap. To address these challenging questions, we will be using lipid mutants (cho1∆, psd1∆ psd2∆…) in Saccharomyces cerevisiae. We are tracking Cdc42 polarity module components in these mutants at the single molecule level by super-resolution imaging in live cells. This system enables the quantification of the diffusion and clustering of individual molecules at the PM, and the contribution that the lipid environment imparts in vivo. In addition, using this powerful system, we will analyze the nano-organization of different phospholipids involved in signaling (PS, PE, P(I4,5)P2, PI4P).

 Publications

year authors and title journal last update
List of publications.
2018 Elodie Sartorel, Caner Ünlü, Mini Jose, Aurélie Massoni-Laporte, Julien Meca, Jean-Baptiste Sibarita, Derek McCusker
Phosphatidylserine and GTPase activation control Cdc42 nanoclustering to counter dissipative diffusion
published pages: mbc.E18-01-0051, ISSN: 1059-1524, DOI: 10.1091/mbc.E18-01-0051
Molecular Biology of the Cell 2019-06-13

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