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Lipid and Polarity SIGNED

The diffusion and nanoclustering of a polarity module in the lipid environment

Total Cost €

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EC-Contrib. €

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Partnership

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 Lipid and Polarity project word cloud

Explore the words cloud of the Lipid and Polarity project. It provides you a very rough idea of what is the project "Lipid and Polarity" about.

pole    activating    embryogenesis    internal    individual    trafficking    though    molecules    pm    gef    cap    dramatic    demonstrated    polarity    proteins    quantification    cerevisiae    vesicle    live    small    leaflet    shown    tracking    bem1    nano    molecule    lipids    play    pe    interact    asymmetrically    ras    mechanisms    imparts    nanoclusters    super    mammalian    contribution    budding    pivotal    localization    phosphatidylserine    cdc42    cellular    lipid    saccharomyces    dynamics    phospholipids    questions    cdc24    cells    regulation    central    organization    establishment    asymmetric    single    imaging    diffusion    polar    entails    pi4p    completely    forms    biology    membranes    universal    ps    diseases    psd1    components    rho    clustering    protein    p2    powerful    cancer    eukaryotic    psd2    signaling    analyze    assembly    localizes    gtpase    cell    vivo    orientation    scaffold    i4    plasma    mutants    resolution    regulator    yeast    environment    cho1    family    influences    membrane    spatial    module    maintenance    function   

Project "Lipid and Polarity" data sheet

The following table provides information about the project.

Coordinator
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS 

Organization address
address: RUE MICHEL ANGE 3
city: PARIS
postcode: 75794
website: www.cnrs.fr

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country France [FR]
 Project website http://ibgc.cnrs.fr/
 Total cost 185˙076 €
 EC max contribution 185˙076 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2015
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2016
 Duration (year-month-day) from 2016-04-01   to  2018-03-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS FR (PARIS) coordinator 185˙076.00

Map

 Project objective

Cellular polarity is a universal feature of eukaryotic biology. Failure to control cell orientation has dramatic consequences on embryogenesis and diseases including cancer. The establishment of cellular polarity often entails the asymmetric distribution of plasma membrane (PM) lipids signaling proteins and vesicle trafficking. The function of Rho proteins in cell polarity has been demonstrated from mammalian cells to yeast. The small GTPase Cdc42 is a pivotal regulator of polarity establishment and maintenance. A central feature of Cdc42 function is its ability to interact with membrane. In budding yeast, Cdc42 localizes asymmetrically at the PM, at the pole of the cell, along with the scaffold protein Bem1 and the Cdc42 activating GEF Cdc24. The asymmetric distribution of lipids in the internal leaflet of the membrane play a crucial role in the regulation of Cdc42 activity and localization, though the mechanisms are not completely understood. The organization of lipids in the PM influences the spatial distribution and activity of key signaling proteins as Ras family proteins. Ras forms nanoclusters in cell membranes that are essential for signaling and Phosphatidylserine lipids have been shown to play a role in the assembly of K-Ras nanoclusters in mammalian cells. In this work, we will study Cdc42, Cdc24 and Bem1 dynamics in the membrane and particularly at the polar cap. To address these challenging questions, we will be using lipid mutants (cho1∆, psd1∆ psd2∆…) in Saccharomyces cerevisiae. We are tracking Cdc42 polarity module components in these mutants at the single molecule level by super-resolution imaging in live cells. This system enables the quantification of the diffusion and clustering of individual molecules at the PM, and the contribution that the lipid environment imparts in vivo. In addition, using this powerful system, we will analyze the nano-organization of different phospholipids involved in signaling (PS, PE, P(I4,5)P2, PI4P).

 Publications

year authors and title journal last update
List of publications.
2018 Elodie Sartorel, Caner Ünlü, Mini Jose, Aurélie Massoni-Laporte, Julien Meca, Jean-Baptiste Sibarita, Derek McCusker
Phosphatidylserine and GTPase activation control Cdc42 nanoclustering to counter dissipative diffusion
published pages: mbc.E18-01-0051, ISSN: 1059-1524, DOI: 10.1091/mbc.E18-01-0051
Molecular Biology of the Cell 2019-06-13

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