Opendata, web and dolomites

Lipid and Polarity SIGNED

The diffusion and nanoclustering of a polarity module in the lipid environment

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 Lipid and Polarity project word cloud

Explore the words cloud of the Lipid and Polarity project. It provides you a very rough idea of what is the project "Lipid and Polarity" about.

membrane    organization    environment    mammalian    gef    polarity    cellular    play    p2    module    central    asymmetric    nanoclusters    single    asymmetrically    gtpase    ras    cancer    diseases    embryogenesis    i4    yeast    cap    demonstrated    shown    imaging    proteins    resolution    function    forms    live    powerful    pe    membranes    rho    though    questions    molecule    biology    analyze    super    spatial    small    contribution    psd2    pivotal    regulator    nano    orientation    lipids    assembly    diffusion    cdc42    cdc24    localizes    ps    components    vesicle    scaffold    pm    quantification    cells    pole    maintenance    leaflet    mechanisms    universal    cell    saccharomyces    cerevisiae    entails    activating    polar    signaling    clustering    plasma    internal    pi4p    tracking    dynamics    protein    phosphatidylserine    psd1    localization    cho1    vivo    establishment    phospholipids    influences    dramatic    imparts    interact    completely    family    molecules    budding    trafficking    individual    regulation    mutants    bem1    lipid    eukaryotic   

Project "Lipid and Polarity" data sheet

The following table provides information about the project.

Coordinator
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS 

Organization address
address: RUE MICHEL ANGE 3
city: PARIS
postcode: 75794
website: www.cnrs.fr

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country France [FR]
 Project website http://ibgc.cnrs.fr/
 Total cost 185˙076 €
 EC max contribution 185˙076 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2015
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2016
 Duration (year-month-day) from 2016-04-01   to  2018-03-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS FR (PARIS) coordinator 185˙076.00

Map

 Project objective

Cellular polarity is a universal feature of eukaryotic biology. Failure to control cell orientation has dramatic consequences on embryogenesis and diseases including cancer. The establishment of cellular polarity often entails the asymmetric distribution of plasma membrane (PM) lipids signaling proteins and vesicle trafficking. The function of Rho proteins in cell polarity has been demonstrated from mammalian cells to yeast. The small GTPase Cdc42 is a pivotal regulator of polarity establishment and maintenance. A central feature of Cdc42 function is its ability to interact with membrane. In budding yeast, Cdc42 localizes asymmetrically at the PM, at the pole of the cell, along with the scaffold protein Bem1 and the Cdc42 activating GEF Cdc24. The asymmetric distribution of lipids in the internal leaflet of the membrane play a crucial role in the regulation of Cdc42 activity and localization, though the mechanisms are not completely understood. The organization of lipids in the PM influences the spatial distribution and activity of key signaling proteins as Ras family proteins. Ras forms nanoclusters in cell membranes that are essential for signaling and Phosphatidylserine lipids have been shown to play a role in the assembly of K-Ras nanoclusters in mammalian cells. In this work, we will study Cdc42, Cdc24 and Bem1 dynamics in the membrane and particularly at the polar cap. To address these challenging questions, we will be using lipid mutants (cho1∆, psd1∆ psd2∆…) in Saccharomyces cerevisiae. We are tracking Cdc42 polarity module components in these mutants at the single molecule level by super-resolution imaging in live cells. This system enables the quantification of the diffusion and clustering of individual molecules at the PM, and the contribution that the lipid environment imparts in vivo. In addition, using this powerful system, we will analyze the nano-organization of different phospholipids involved in signaling (PS, PE, P(I4,5)P2, PI4P).

 Publications

year authors and title journal last update
List of publications.
2018 Elodie Sartorel, Caner Ünlü, Mini Jose, Aurélie Massoni-Laporte, Julien Meca, Jean-Baptiste Sibarita, Derek McCusker
Phosphatidylserine and GTPase activation control Cdc42 nanoclustering to counter dissipative diffusion
published pages: mbc.E18-01-0051, ISSN: 1059-1524, DOI: 10.1091/mbc.E18-01-0051
Molecular Biology of the Cell 2019-06-13

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "LIPID AND POLARITY" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "LIPID AND POLARITY" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.3.2.)

Photonic Radar (2019)

Implementation of Long Reach Hybrid Photonic Radar System and convergence over FSO and PON Networks

Read More  

DNANanoProbes (2019)

Design of light-harvesting DNA-nanoprobes with ratiometric signal amplification for fluorescence imaging of live cells.

Read More  

MarshFlux (2020)

The effect of future global climate and land-use change on greenhouse gas fluxes and microbial processes in salt marshes

Read More