Opendata, web and dolomites

CHROMTOPOLOGY SIGNED

Understanding and manipulating the dynamics of chromosome topologies in transcriptional control

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 CHROMTOPOLOGY project word cloud

Explore the words cloud of the CHROMTOPOLOGY project. It provides you a very rough idea of what is the project "CHROMTOPOLOGY" about.

tagging    shown    contact    chromatin    regulates    direct    view    ins    regulation    domains    engineer    organization    understand    correlation    crispr    sequences    hi    variabilities    folded    tad    loops    replication    combination    genomes    packaged    novo    sized    kinetics    anticipate    interplay    de    epigenetic    super    regions    megabase    optogenetics    consequence    dna    development    altered    unknown    knock    enhancers    influence    screens    accompanying    seem    questions    topology    expression    time    gene    loci    discretely    genetic    give    tightly    topologically    function    environment    anchor    denoted    chromosome    eukaryotic    configurations    single    genes    textbook    imaging    paradigm    tads    organize    repair    metazoan    regulatory    accessible    fundamental    exists    interactions    transcription    groundbreaking    modifications    histone    trans    underlying    resolution    striking    correlate    architectures    genome    distal    acting    transcriptional    cell    cells    regulated    technologies    engineered    status    folding    nuclei   

Project "CHROMTOPOLOGY" data sheet

The following table provides information about the project.

Coordinator
CENTRE EUROPEEN DE RECHERCHE EN BIOLOGIE ET MEDECINE 

Organization address
address: Rue Laurent Fries 1
city: ILLKIRCH GRAFFENSTADEN
postcode: 67404
website: www.igbmc.fr

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country France [FR]
 Project website http://www.igbmc.fr/research/department/2/team/118/
 Total cost 1˙500˙000 €
 EC max contribution 1˙500˙000 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2015-STG
 Funding Scheme ERC-STG
 Starting year 2016
 Duration (year-month-day) from 2016-06-01   to  2021-05-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    CENTRE EUROPEEN DE RECHERCHE EN BIOLOGIE ET MEDECINE FR (ILLKIRCH GRAFFENSTADEN) coordinator 1˙500˙000.00

Map

 Project objective

Transcriptional regulation of genes in eukaryotic cells requires a complex and highly regulated interplay of chromatin environment, epigenetic status of target sequences and several different transcription factors. Eukaryotic genomes are tightly packaged within nuclei, yet must be accessible for transcription, replication and repair. A striking correlation exists between chromatin topology and underlying gene activity. According to the textbook view, chromatin loops bring genes into direct contact with distal regulatory elements, such as enhancers. Moreover, we and others have shown that genomes are organized into discretely folded megabase-sized regions, denoted as topologically associated domains (TADs), which seem to correlate well with transcription activity and histone modifications. However, it is unknown whether chromosome folding is a cause or consequence of underlying gene function. To better understand the role of genome organization in transcription regulation, I will address the following questions:

(i) How are chromatin configurations altered during transcriptional changes accompanying development? (ii) What are the real-time kinetics and cell-to-cell variabilities of chromatin interactions and TAD architectures? (iii) Can chromatin loops be engineered de novo, and do they influence gene expression? (iv) What genetic elements and trans-acting factors are required to organize TADs?

To address these fundamental questions, I will use a combination of novel technologies and approaches, such as Hi-C, CRISPR knock-ins, ANCHOR tagging of DNA loci, high- and super-resolution single-cell imaging, genome-wide screens and optogenetics, in order to both study and engineer chromatin architectures. These studies will give groundbreaking insight into if and how chromatin topology regulates transcription. Thus, I anticipate that the results of this project will have a major impact on the field and will lead to a new paradigm for metazoan transcription control.

 Publications

year authors and title journal last update
List of publications.
2020 Yousra Ben Zouari, Angeliki Platania, Anne M. Molitor, Tom Sexton
4See: A Flexible Browser to Explore 4C Data
published pages: , ISSN: 1664-8021, DOI: 10.3389/fgene.2019.01372
Frontiers in Genetics 10 2020-03-11
2019 Yousra Ben Zouari, Anne M. Molitor, Natalia Sikorska, Vera Pancaldi, Tom Sexton
ChiCMaxima: a robust and simple pipeline for detection and visualization of chromatin looping in Capture Hi-C
published pages: , ISSN: 1474-760X, DOI: 10.1186/s13059-019-1706-3
Genome Biology 20/1 2019-08-30

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "CHROMTOPOLOGY" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "CHROMTOPOLOGY" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.1.)

MitoGuide (2019)

Integration and adaptation of impaired mitochondrial fitness in orchestrating T cell dysfunction in the tumor microenvironment

Read More  

EVOMENS (2020)

The evolution of menstruation in primates

Read More  

NanoPD_P (2020)

High throughput multiplexed trace-analyte screening for diagnostics applications

Read More