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Investigation of 3D Functional Connectivity in Mouse Visual Cortex using Holography and Optogenetics

Total Cost €


EC-Contrib. €






 3DMUCHO project word cloud

Explore the words cloud of the 3DMUCHO project. It provides you a very rough idea of what is the project "3DMUCHO" about.

laboratory    tuning    insights    selectivity    convergent    fellowship    innovative    sharing    determined    neuroscience    light    brain    neuron    unresolved    underlying    cortex    photon    connectivity    resolved    connection    patterning    single    mouse    calcium    pairs    spanning    horizontally    interdisciplinary    toolbox    pioneered    optical    probe    subnetwork    population    combined    cell    attributes    me    vivo    dimensional    superficial    employing    volume    3d    relationship    network    linearity    individual    simultaneous    enabled    time    excitation    holography    acquire    neuronal    deep    vertically    optogenetics    permit    render    presynaptic    cortical    mechanism    sensory    imaging    precise    circuit    first    specificity    connect    selectively    mapping    visual    versatile    examined    uncover    evidences    point    neurons    holographic    manipulation    layers    measuring    local    recordings    postsynaptic    planes    functional    integration    connecting    functionally    host    activation    stronger   

Project "3DMUCHO" data sheet

The following table provides information about the project.


Organization address
address: RUE MICHEL ANGE 3
city: PARIS
postcode: 75794

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country France [FR]
 Total cost 173˙076 €
 EC max contribution 173˙076 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2016
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2017
 Duration (year-month-day) from 2017-04-01   to  2019-03-31


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 


 Project objective

Cortical neurons respond selectively to sensory features. In mouse visual cortex, convergent evidences point towards stronger connectivity between neurons sharing similar feature selectivity in the superficial layers. The relationship between a three-dimensional (3D) subnetwork, where neurons connect horizontally and vertically to each other, and the functional specificity of individual neurons remain however unresolved. The main objective of this interdisciplinary project is to uncover the 3D functional connectivity in mouse visual cortex in vivo by employing the innovative optical methods pioneered in the host laboratory. Single-cell activation deep into the brain will be, for the first time, enabled using the novel technology of 3D depth-resolved holographic light-patterning and two-photon optogenetics. Combined with whole-cell recordings for measuring postsynaptic responses, precise presynaptic excitation with holography will permit mapping connection pairs within and across cortical planes. Simultaneous presynaptic multi-cell excitation of connecting neurons will test the integration linearity of the postsynaptic neuron. To probe functional connectivity, connection pairs will be examined from a functionally-identified neuronal population, whose tuning specificity to visual attributes is determined using calcium imaging. This fellowship will render me opportunities to acquire a versatile optical toolbox that is of general interest in neuroscience for connectivity mapping and circuit manipulation. Knowledge of local functional connectivity spanning a 3D cortical volume will further provide significant insights into the network mechanism underlying sensory processing.


year authors and title journal last update
List of publications.
2018 I-Wen Chen, Eirini Papagiakoumou, Valentina Emiliani
Towards circuit optogenetics
published pages: 179-189, ISSN: 0959-4388, DOI: 10.1016/j.conb.2018.03.008
Current Opinion in Neurobiology 50 2019-09-25
2019 I-Wen Chen, Emiliano Ronzitti, Brian R. Lee, Tanya L. Daigle, Deniz Dalkara, Hongkui Zeng, Valentina Emiliani, Eirini Papagiakoumou
In vivo sub-millisecond two-photon optogenetics with temporally focused patterned light
published pages: 1785-18, ISSN: 0270-6474, DOI: 10.1523/jneurosci.1785-18.2018
The Journal of Neuroscience 2019-09-25

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