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Investigation of 3D Functional Connectivity in Mouse Visual Cortex using Holography and Optogenetics

Total Cost €


EC-Contrib. €






 3DMUCHO project word cloud

Explore the words cloud of the 3DMUCHO project. It provides you a very rough idea of what is the project "3DMUCHO" about.

cortical    superficial    first    network    connectivity    host    holography    point    acquire    sensory    dimensional    layers    activation    interdisciplinary    presynaptic    underlying    resolved    manipulation    deep    selectivity    measuring    evidences    photon    patterning    mouse    neuron    excitation    linearity    imaging    neurons    local    recordings    render    examined    uncover    volume    innovative    connect    enabled    light    mapping    cortex    time    simultaneous    tuning    individual    pairs    toolbox    postsynaptic    neuronal    optical    unresolved    3d    vertically    calcium    planes    neuroscience    attributes    visual    precise    sharing    determined    stronger    permit    relationship    integration    cell    mechanism    combined    functionally    me    versatile    specificity    optogenetics    connection    circuit    fellowship    brain    subnetwork    functional    vivo    laboratory    convergent    single    horizontally    spanning    insights    holographic    population    pioneered    connecting    employing    selectively    probe   

Project "3DMUCHO" data sheet

The following table provides information about the project.


Organization address
address: RUE MICHEL ANGE 3
city: PARIS
postcode: 75794

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country France [FR]
 Total cost 173˙076 €
 EC max contribution 173˙076 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2016
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2017
 Duration (year-month-day) from 2017-04-01   to  2019-03-31


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 


 Project objective

Cortical neurons respond selectively to sensory features. In mouse visual cortex, convergent evidences point towards stronger connectivity between neurons sharing similar feature selectivity in the superficial layers. The relationship between a three-dimensional (3D) subnetwork, where neurons connect horizontally and vertically to each other, and the functional specificity of individual neurons remain however unresolved. The main objective of this interdisciplinary project is to uncover the 3D functional connectivity in mouse visual cortex in vivo by employing the innovative optical methods pioneered in the host laboratory. Single-cell activation deep into the brain will be, for the first time, enabled using the novel technology of 3D depth-resolved holographic light-patterning and two-photon optogenetics. Combined with whole-cell recordings for measuring postsynaptic responses, precise presynaptic excitation with holography will permit mapping connection pairs within and across cortical planes. Simultaneous presynaptic multi-cell excitation of connecting neurons will test the integration linearity of the postsynaptic neuron. To probe functional connectivity, connection pairs will be examined from a functionally-identified neuronal population, whose tuning specificity to visual attributes is determined using calcium imaging. This fellowship will render me opportunities to acquire a versatile optical toolbox that is of general interest in neuroscience for connectivity mapping and circuit manipulation. Knowledge of local functional connectivity spanning a 3D cortical volume will further provide significant insights into the network mechanism underlying sensory processing.


year authors and title journal last update
List of publications.
2018 I-Wen Chen, Eirini Papagiakoumou, Valentina Emiliani
Towards circuit optogenetics
published pages: 179-189, ISSN: 0959-4388, DOI: 10.1016/j.conb.2018.03.008
Current Opinion in Neurobiology 50 2019-09-25
2019 I-Wen Chen, Emiliano Ronzitti, Brian R. Lee, Tanya L. Daigle, Deniz Dalkara, Hongkui Zeng, Valentina Emiliani, Eirini Papagiakoumou
In vivo sub-millisecond two-photon optogenetics with temporally focused patterned light
published pages: 1785-18, ISSN: 0270-6474, DOI: 10.1523/jneurosci.1785-18.2018
The Journal of Neuroscience 2019-09-25

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