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OMPorg SIGNED

Spatiotemporal organisation of bacterial outer membrane proteins

Total Cost €

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EC-Contrib. €

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Partnership

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 OMPorg project word cloud

Explore the words cloud of the OMPorg project. It provides you a very rough idea of what is the project "OMPorg" about.

dynamics    purified    questions    tools    interdisciplinary    displaced    live    protects    mimicked    cell    single    turned    bacterial    influence    ensemble    immobility    impacts    im    microscopy    clustering    caused    laboratory    om    integrity    memory    protein    discovered    molecular    underpin    organising    bridges    periplasm    environmental    cells    envelope    bilayers    becomes    grow    limited    island    inner    connected    gram    omporg    answer    incorporating    binary    interrelated    biomedicine    imps    bacteria    islands    largely    molecule    biotechnology    species    populations    spatiotemporal    explains    ramifications    vivo    septation    poorly    simulations    negative    outstanding    aeruginosa    found    mediate    processes    ways    escherichia    ask    coli    omp    biology    cluster    repository    route    hence    coordinate    membrane    unknown    partitioning    omps    chemistry    outer    antibiotics    concerning    imp    disrupt    poles    central    endow    basis    imposed    functions    proteins    organisation    insult    pathogenesis    connect    formation    proteomics   

Project "OMPorg" data sheet

The following table provides information about the project.

Coordinator
THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD 

Organization address
address: WELLINGTON SQUARE UNIVERSITY OFFICES
city: OXFORD
postcode: OX1 2JD
website: www.ox.ac.uk

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Total cost 2˙235˙815 €
 EC max contribution 2˙235˙815 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2016-ADG
 Funding Scheme ERC-ADG
 Starting year 2017
 Duration (year-month-day) from 2017-09-01   to  2022-08-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD UK (OXFORD) coordinator 2˙235˙815.00

Map

 Project objective

The outer membrane (OM) of Gram-negative bacteria protects against environmental insult and is central to pathogenesis hence finding ways to disrupt its integrity is a route towards new antibiotics. However, our understanding of OM biology is limited. In particular, how the OM is organised is largely unknown. My laboratory recently discovered spatiotemporal Outer Membrane Protein organisation (OMPorg) in Escherichia coli, a new organising principle that explains how OMPs are turned over. We found that OMPs cluster into islands that can be mimicked in supported bilayers using purified proteins. As cells grow, OMP islands are displaced to the poles by new islands, leading to binary partitioning of old OMPs in repository cells following septation.

Another poorly understood aspect of OM biology is how protein bridges that connect the OM to the inner membrane (IM) mediate functions across the periplasm. We have discovered that the characteristic immobility of OMPs in vivo, caused by OMP clustering, becomes imposed on IM proteins (IMPs) when OMPs and IMPs become connected by protein bridges.

These exciting new findings underpin OMPorg. I will focus on two species, E. coli and P. aeruginosa, and exploit the tools I have developed to ask four interrelated questions:

1. What is the molecular basis of OMP island formation? 2. Do OMPs influence IMP functionality via protein bridges? 3. Do repository cells endow bacterial populations with ‘OMP memory’? 4. Do OMP islands coordinate OM processes?

I will address these questions through an interdisciplinary research programme incorporating protein chemistry and proteomics, ensemble and single molecule microscopy on live cells and in supported bilayers and molecular dynamics simulations. OMPorg will answer major outstanding questions concerning organisation in the bacterial OM and how this impacts the biology of the cell envelope, which will have ramifications for biomedicine and biotechnology.

 Publications

year authors and title journal last update
List of publications.
2018 Patrice Rassam, Kathleen R. Long, Renata Kaminska, David J. Williams, Grigorios Papadakos, Christoph G. Baumann, Colin Kleanthous
Intermembrane crosstalk drives inner-membrane protein organization in Escherichia coli
published pages: , ISSN: 2041-1723, DOI: 10.1038/s41467-018-03521-4
Nature Communications 9/1 2019-04-18
2018 Matthieu Chavent, Anna L. Duncan, Patrice Rassam, Oliver Birkholz, Jean Hélie, Tyler Reddy, Dmitry Beliaev, Ben Hambly, Jacob Piehler, Colin Kleanthous, Mark S. P. Sansom
How nanoscale protein interactions determine the mesoscale dynamic organisation of bacterial outer membrane proteins
published pages: , ISSN: 2041-1723, DOI: 10.1038/s41467-018-05255-9
Nature Communications 9/1 2019-04-18
2019 Connor Sharp, Christine Boinett, Amy Cain, Nicholas G. Housden, Sandip Kumar, Keith Turner, Julian Parkhill, Colin Kleanthous
O-Antigen-Dependent Colicin Insensitivity of Uropathogenic Escherichia coli
published pages: , ISSN: 0021-9193, DOI: 10.1128/jb.00545-18
Journal of Bacteriology 201/4 2019-04-16

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The information about "OMPORG" are provided by the European Opendata Portal: CORDIS opendata.

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