Opendata, web and dolomites

OMPorg SIGNED

Spatiotemporal organisation of bacterial outer membrane proteins

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 OMPorg project word cloud

Explore the words cloud of the OMPorg project. It provides you a very rough idea of what is the project "OMPorg" about.

proteomics    cell    om    processes    islands    pathogenesis    envelope    incorporating    dynamics    proteins    caused    omp    limited    underpin    formation    single    turned    clustering    explains    ramifications    ask    connect    tools    mimicked    grow    escherichia    connected    omporg    aeruginosa    molecular    organising    biomedicine    environmental    imposed    interrelated    found    biology    unknown    gram    displaced    basis    negative    microscopy    populations    biotechnology    bacterial    poorly    impacts    mediate    outer    questions    molecule    repository    route    laboratory    antibiotics    bacteria    disrupt    inner    interdisciplinary    septation    periplasm    concerning    purified    island    spatiotemporal    integrity    answer    largely    ensemble    discovered    protects    partitioning    endow    imp    outstanding    protein    omps    coli    chemistry    im    central    becomes    bridges    memory    immobility    functions    organisation    binary    influence    cells    bilayers    vivo    species    simulations    insult    coordinate    live    hence    imps    cluster    poles    membrane    ways   

Project "OMPorg" data sheet

The following table provides information about the project.

Coordinator
THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD 

Organization address
address: WELLINGTON SQUARE UNIVERSITY OFFICES
city: OXFORD
postcode: OX1 2JD
website: www.ox.ac.uk

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Total cost 2˙235˙815 €
 EC max contribution 2˙235˙815 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2016-ADG
 Funding Scheme ERC-ADG
 Starting year 2017
 Duration (year-month-day) from 2017-09-01   to  2022-08-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD UK (OXFORD) coordinator 2˙235˙815.00

Map

 Project objective

The outer membrane (OM) of Gram-negative bacteria protects against environmental insult and is central to pathogenesis hence finding ways to disrupt its integrity is a route towards new antibiotics. However, our understanding of OM biology is limited. In particular, how the OM is organised is largely unknown. My laboratory recently discovered spatiotemporal Outer Membrane Protein organisation (OMPorg) in Escherichia coli, a new organising principle that explains how OMPs are turned over. We found that OMPs cluster into islands that can be mimicked in supported bilayers using purified proteins. As cells grow, OMP islands are displaced to the poles by new islands, leading to binary partitioning of old OMPs in repository cells following septation.

Another poorly understood aspect of OM biology is how protein bridges that connect the OM to the inner membrane (IM) mediate functions across the periplasm. We have discovered that the characteristic immobility of OMPs in vivo, caused by OMP clustering, becomes imposed on IM proteins (IMPs) when OMPs and IMPs become connected by protein bridges.

These exciting new findings underpin OMPorg. I will focus on two species, E. coli and P. aeruginosa, and exploit the tools I have developed to ask four interrelated questions:

1. What is the molecular basis of OMP island formation? 2. Do OMPs influence IMP functionality via protein bridges? 3. Do repository cells endow bacterial populations with ‘OMP memory’? 4. Do OMP islands coordinate OM processes?

I will address these questions through an interdisciplinary research programme incorporating protein chemistry and proteomics, ensemble and single molecule microscopy on live cells and in supported bilayers and molecular dynamics simulations. OMPorg will answer major outstanding questions concerning organisation in the bacterial OM and how this impacts the biology of the cell envelope, which will have ramifications for biomedicine and biotechnology.

 Publications

year authors and title journal last update
List of publications.
2018 Patrice Rassam, Kathleen R. Long, Renata Kaminska, David J. Williams, Grigorios Papadakos, Christoph G. Baumann, Colin Kleanthous
Intermembrane crosstalk drives inner-membrane protein organization in Escherichia coli
published pages: , ISSN: 2041-1723, DOI: 10.1038/s41467-018-03521-4
Nature Communications 9/1 2019-04-18
2018 Matthieu Chavent, Anna L. Duncan, Patrice Rassam, Oliver Birkholz, Jean Hélie, Tyler Reddy, Dmitry Beliaev, Ben Hambly, Jacob Piehler, Colin Kleanthous, Mark S. P. Sansom
How nanoscale protein interactions determine the mesoscale dynamic organisation of bacterial outer membrane proteins
published pages: , ISSN: 2041-1723, DOI: 10.1038/s41467-018-05255-9
Nature Communications 9/1 2019-04-18
2019 Connor Sharp, Christine Boinett, Amy Cain, Nicholas G. Housden, Sandip Kumar, Keith Turner, Julian Parkhill, Colin Kleanthous
O-Antigen-Dependent Colicin Insensitivity of Uropathogenic Escherichia coli
published pages: , ISSN: 0021-9193, DOI: 10.1128/jb.00545-18
Journal of Bacteriology 201/4 2019-04-16

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "OMPORG" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "OMPORG" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.1.)

CITISENSE (2019)

Evolving communication systems in response to altered sensory environments

Read More  

T-NHL SUPRESSORS (2019)

Tumor suppressor pathways counteracting oncogenic immune receptor signaling in T-Cell Lymphoma

Read More  

NanoPD_P (2020)

High throughput multiplexed trace-analyte screening for diagnostics applications

Read More