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EpiTrack SIGNED

Single-cell temporal tracking of epigenetic DNA marks

Total Cost €

0

EC-Contrib. €

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Partnership

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 EpiTrack project word cloud

Explore the words cloud of the EpiTrack project. It provides you a very rough idea of what is the project "EpiTrack" about.

sites    extensively    epigenomic    dynamics    phenomena    single    methyltransferases    barcoding    imaging    dramatic    horizons    mammals    groups    expression    metabolically    chemical    mapping    over    stage    answered    gap    neural    reprogramming    fertilization    prevalent    mammalian    unprecedented    transfer    genomic    cellular    vivo    methylation    profiling    whereby    disease    questions    massive    super    lineages    epigenetic    give    microdroplet    time    events    occurs    individual    decade    experimental    variability    pulse    obscure    mouse    global    tagging    analog    mechanistic    plasticity    patterns    followed    dna    resolution    embryonic    deposited    differentiation    modification    cell    innovative    cells    cofactor    enzymatic    marks    bridge    permit    reinstatement    regulation    modifications    precise    centre    dnmts    fascinating    sam    methyl    play    roles    gene    view    govern    resolved    integration    human    engineer    throughput    adenosylmethionine   

Project "EpiTrack" data sheet

The following table provides information about the project.

Coordinator
VILNIAUS UNIVERSITETAS 

Organization address
address: UNIVERSITETO G. 3
city: VILNIUS
postcode: 1513
website: http://www.vu.lt

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Lithuania [LT]
 Total cost 2˙499˙875 €
 EC max contribution 2˙499˙875 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2016-ADG
 Funding Scheme ERC-ADG
 Starting year 2017
 Duration (year-month-day) from 2017-09-01   to  2022-08-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    VILNIAUS UNIVERSITETAS LT (VILNIUS) coordinator 2˙499˙875.00

Map

 Project objective

Over the past decade, epigenetic phenomena have taken centre stage in our understanding of gene regulation, cellular differentiation and human disease. DNA methylation is a prevalent epigenetic modification in mammals, which is brought about by enzymatic transfer of methyl groups from the S-adenosylmethionine (SAM) cofactor by three known DNA methyltransferases (DNMTs). The most dramatic epigenomic reprogramming in mammalian development occurs after fertilization, whereby a global loss of DNA methylation is followed by massive reinstatement of new methylation patterns, different for each cell type. Although DNA methylation has been extensively investigated, key mechanistic aspects of these fascinating events remain obscure. The goal of this proposal is to bridge the gap in our understanding of how the genomic methylation patterns are established and how they govern cell plasticity and variability during differentiation and development. These questions could only be answered by precise determination of where and when methylation marks are deposited by the individual DNMTs, and how these methylation marks affect gene expression. To achieve this ambitious goal, we will metabolically engineer mouse cells to permit SAM analog-based chemical pulse-tagging of their methylation sites in vivo. We will then advance profiling of DNA modifications to the single cell level via innovative integration of microdroplet-based barcoding, precise genomic mapping and super-resolution imaging. Using this unique experimental system we will determine, with unprecedented detail and throughput, the dynamics and variability of DNA methylation and gene expression patterns during differentiation of mouse embryonic cells to neural and other lineages. This project will give a comprehensive, time-resolved view of the roles that the DNMTs play in mammalian development, which will open new horizons in epigenomic research and will advance our understanding of human development and disease.

 Publications

year authors and title journal last update
List of publications.
2020 Povilas Gibas, Milda Narmontė, Zdislav Staševskij, Juozas Gordevičius, Saulius Klimašauskas, Edita Kriukienė
Precise genomic mapping of 5-hydroxymethylcytosine via covalent tether-directed sequencing
published pages: e3000684, ISSN: 1544-9173, DOI: 10.1371/journal.pbio.3000684
PLOS Biology 18 2020-04-15
2018 Milda Mickutė, Milda Nainytė, Lina Vasiliauskaitė, Alexandra Plotnikova, Viktoras Masevičius, Saulius Klimašauskas, Giedrius Vilkaitis
Animal Hen1 2′-O-methyltransferases as tools for 3′-terminal functionalization and labelling of single-stranded RNAs
published pages: e104-e104, ISSN: 0305-1048, DOI: 10.1093/nar/gky514
Nucleic Acids Research 46/17 2019-04-18
2019 Saulius Klimašauskas, Linas Mažutis
Editorial overview: Current advances in analytical biotechnology: from single molecules to whole organisms
published pages: , ISSN: 0958-1669, DOI: 10.1016/j.copbio.2018.12.006
Current Opinion in Biotechnology 2019-02-28
2019 Miglė Tomkuvienė, Milda Mickutė, Giedrius Vilkaitis, Saulius Klimašauskas
Repurposing enzymatic transferase reactions for targeted labeling and analysis of DNA and RNA
published pages: 114-123, ISSN: 0958-1669, DOI: 10.1016/j.copbio.2018.09.008
Current Opinion in Biotechnology 55 2019-02-12

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