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EpiTrack SIGNED

Single-cell temporal tracking of epigenetic DNA marks

Total Cost €

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EC-Contrib. €

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Partnership

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 EpiTrack project word cloud

Explore the words cloud of the EpiTrack project. It provides you a very rough idea of what is the project "EpiTrack" about.

super    resolved    barcoding    permit    methylation    modifications    fascinating    dramatic    deposited    bridge    resolution    cofactor    play    give    reprogramming    marks    horizons    prevalent    pulse    throughput    plasticity    time    patterns    stage    mouse    precise    decade    gap    reinstatement    mechanistic    sam    whereby    dna    methyltransferases    answered    individual    expression    neural    dynamics    followed    embryonic    imaging    obscure    genomic    gene    innovative    metabolically    integration    modification    disease    govern    fertilization    epigenomic    experimental    profiling    cell    methyl    differentiation    regulation    single    chemical    dnmts    transfer    mammalian    epigenetic    roles    centre    occurs    vivo    microdroplet    view    human    extensively    sites    mammals    groups    massive    enzymatic    cells    unprecedented    variability    events    cellular    phenomena    adenosylmethionine    mapping    lineages    global    over    questions    engineer    analog    tagging   

Project "EpiTrack" data sheet

The following table provides information about the project.

Coordinator
VILNIAUS UNIVERSITETAS 

Organization address
address: UNIVERSITETO G. 3
city: VILNIUS
postcode: 1513
website: http://www.vu.lt

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
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 Coordinator Country Lithuania [LT]
 Total cost 2˙499˙875 €
 EC max contribution 2˙499˙875 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2016-ADG
 Funding Scheme ERC-ADG
 Starting year 2017
 Duration (year-month-day) from 2017-09-01   to  2022-08-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    VILNIAUS UNIVERSITETAS LT (VILNIUS) coordinator 2˙499˙875.00

Map

 Project objective

Over the past decade, epigenetic phenomena have taken centre stage in our understanding of gene regulation, cellular differentiation and human disease. DNA methylation is a prevalent epigenetic modification in mammals, which is brought about by enzymatic transfer of methyl groups from the S-adenosylmethionine (SAM) cofactor by three known DNA methyltransferases (DNMTs). The most dramatic epigenomic reprogramming in mammalian development occurs after fertilization, whereby a global loss of DNA methylation is followed by massive reinstatement of new methylation patterns, different for each cell type. Although DNA methylation has been extensively investigated, key mechanistic aspects of these fascinating events remain obscure. The goal of this proposal is to bridge the gap in our understanding of how the genomic methylation patterns are established and how they govern cell plasticity and variability during differentiation and development. These questions could only be answered by precise determination of where and when methylation marks are deposited by the individual DNMTs, and how these methylation marks affect gene expression. To achieve this ambitious goal, we will metabolically engineer mouse cells to permit SAM analog-based chemical pulse-tagging of their methylation sites in vivo. We will then advance profiling of DNA modifications to the single cell level via innovative integration of microdroplet-based barcoding, precise genomic mapping and super-resolution imaging. Using this unique experimental system we will determine, with unprecedented detail and throughput, the dynamics and variability of DNA methylation and gene expression patterns during differentiation of mouse embryonic cells to neural and other lineages. This project will give a comprehensive, time-resolved view of the roles that the DNMTs play in mammalian development, which will open new horizons in epigenomic research and will advance our understanding of human development and disease.

 Publications

year authors and title journal last update
List of publications.
2020 Povilas Gibas, Milda Narmontė, Zdislav Staševskij, Juozas Gordevičius, Saulius Klimašauskas, Edita Kriukienė
Precise genomic mapping of 5-hydroxymethylcytosine via covalent tether-directed sequencing
published pages: e3000684, ISSN: 1544-9173, DOI: 10.1371/journal.pbio.3000684
PLOS Biology 18 2020-04-15
2018 Milda Mickutė, Milda Nainytė, Lina Vasiliauskaitė, Alexandra Plotnikova, Viktoras Masevičius, Saulius Klimašauskas, Giedrius Vilkaitis
Animal Hen1 2′-O-methyltransferases as tools for 3′-terminal functionalization and labelling of single-stranded RNAs
published pages: e104-e104, ISSN: 0305-1048, DOI: 10.1093/nar/gky514
Nucleic Acids Research 46/17 2019-04-18
2019 Saulius Klimašauskas, Linas Mažutis
Editorial overview: Current advances in analytical biotechnology: from single molecules to whole organisms
published pages: , ISSN: 0958-1669, DOI: 10.1016/j.copbio.2018.12.006
Current Opinion in Biotechnology 2019-02-28
2019 Miglė Tomkuvienė, Milda Mickutė, Giedrius Vilkaitis, Saulius Klimašauskas
Repurposing enzymatic transferase reactions for targeted labeling and analysis of DNA and RNA
published pages: 114-123, ISSN: 0958-1669, DOI: 10.1016/j.copbio.2018.09.008
Current Opinion in Biotechnology 55 2019-02-12

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