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MICROCRYO SIGNED

Microsystems for Cryomicroscopy

Total Cost €

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EC-Contrib. €

0

Partnership

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 MICROCRYO project word cloud

Explore the words cloud of the MICROCRYO project. It provides you a very rough idea of what is the project "MICROCRYO" about.

modes    events    powerful    decade    elucidate    biological    incrementally    trafficking    division    warming    microscopy    spatial    light    structural    create    basis    emerged    live    membrane    unperturbed    ultrastructural    breakthroughs    synaptic    microsystems    dynamics    infancy    cryo    fast    millisecond    resolved    sperm    optics    stem    dynamic    systematically    time    cell    technologies    proteins    reversed    combined    transmission    cells    revealing    imaged    untapped    organisms    extremely    function    transport    sophisticated    nanometer    class    single    synergy    ultimately    preparation    limitations    cryofixation    instrumentation    temperature    cryopreservation    electron    reversible    revolutionize    model    1960s    oocytes    resume    vitrification    small    molecular    microscope    reversibility    microfluidic    first    cooling    cellular    cryogenic    progress    despite    ultra    direct    structure    resolution    context    imaging    rapid    heating    temporal   

Project "MICROCRYO" data sheet

The following table provides information about the project.

Coordinator
TECHNISCHE UNIVERSITAT DARMSTADT 

Organization address
address: KAROLINENPLATZ 5
city: DARMSTADT
postcode: 64289
website: www.tu-darmstadt.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 1˙994˙562 €
 EC max contribution 1˙994˙562 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2017-COG
 Funding Scheme ERC-COG
 Starting year 2019
 Duration (year-month-day) from 2019-02-01   to  2024-01-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    TECHNISCHE UNIVERSITAT DARMSTADT DE (DARMSTADT) coordinator 1˙994˙562.00
2    MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV DE (MUENCHEN) participant 0.00

Map

 Project objective

This proposal aims to revolutionize time-resolved light and electron cryo-microscopy of fast cellular dynamics using a new class of cryogenic microsystems for the reversible cryofixation of cells and small model organisms by ultra-rapid cooling. This will contribute to our understanding of biological structure and function by revealing the dynamics of specific proteins in the ultrastructural context of a cell at nanometer spatial and millisecond temporal resolution.

Despite rapid progress in the field, much of the potential of microscopy at cryogenic temperature today is still untapped due to limitations in methods and instrumentation for sample preparation. First, vitrification technologies for cryo-microscopy have evolved only incrementally since the 1960s and cannot be combined with many of the sophisticated live imaging methods that have emerged over the past decade. Second, while the synergy of light and electron cryo-microscopy is extremely powerful, cryo-microscopy with light is still in its infancy. Finally, new technologies for ultra-rapid heating and cooling of single cells are needed to systematically advance our understanding of reversibility in the cryopreservation of e.g. stem cells, oocytes, or sperm cells. Here I propose to create a microfluidic technology for the direct vitrification of cells in the light microscope by ultra-rapid cooling with millisecond time resolution. The cells will then be imaged at high resolution using electron microscopy and advanced modes of light microscopy combined with new optics adapted to cryogenic conditions. Ultimately, we will elucidate if and under which conditions cryofixation can be reversed by ultra-rapid warming such that dynamic cellular processes resume unperturbed.

We expect that the research proposed here will enable breakthroughs in understanding the structural and molecular basis of fast cellular events including transport, membrane trafficking, cell division, and synaptic transmission.

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The information about "MICROCRYO" are provided by the European Opendata Portal: CORDIS opendata.

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