MICROCRYO SIGNED
Microsystems for Cryomicroscopy
Total Cost €
0EC-Contrib. €
0Partnership
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0MICROCRYO project word cloud
Explore the words cloud of the MICROCRYO project. It provides you a very rough idea of what is the project "MICROCRYO" about.
Project "MICROCRYO" data sheet
The following table provides information about the project.
| Coordinator |
TECHNISCHE UNIVERSITAT DARMSTADT
Organization address contact info |
| Coordinator Country | Germany [DE] |
| Total cost | 1˙994˙562 € |
| EC max contribution | 1˙994˙562 € (100%) |
| Programme |
1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC)) |
| Code Call | ERC-2017-COG |
| Funding Scheme | ERC-COG |
| Starting year | 2019 |
| Duration (year-month-day) | from 2019-02-01 to 2024-01-31 |
Partnership
Take a look of project's partnership.
| # | ||||
|---|---|---|---|---|
| 1 | TECHNISCHE UNIVERSITAT DARMSTADT | DE (DARMSTADT) | coordinator | 1˙994˙562.00 |
| 2 | MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV | DE (MUENCHEN) | participant | 0.00 |
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Project objective
This proposal aims to revolutionize time-resolved light and electron cryo-microscopy of fast cellular dynamics using a new class of cryogenic microsystems for the reversible cryofixation of cells and small model organisms by ultra-rapid cooling. This will contribute to our understanding of biological structure and function by revealing the dynamics of specific proteins in the ultrastructural context of a cell at nanometer spatial and millisecond temporal resolution.
Despite rapid progress in the field, much of the potential of microscopy at cryogenic temperature today is still untapped due to limitations in methods and instrumentation for sample preparation. First, vitrification technologies for cryo-microscopy have evolved only incrementally since the 1960s and cannot be combined with many of the sophisticated live imaging methods that have emerged over the past decade. Second, while the synergy of light and electron cryo-microscopy is extremely powerful, cryo-microscopy with light is still in its infancy. Finally, new technologies for ultra-rapid heating and cooling of single cells are needed to systematically advance our understanding of reversibility in the cryopreservation of e.g. stem cells, oocytes, or sperm cells. Here I propose to create a microfluidic technology for the direct vitrification of cells in the light microscope by ultra-rapid cooling with millisecond time resolution. The cells will then be imaged at high resolution using electron microscopy and advanced modes of light microscopy combined with new optics adapted to cryogenic conditions. Ultimately, we will elucidate if and under which conditions cryofixation can be reversed by ultra-rapid warming such that dynamic cellular processes resume unperturbed.
We expect that the research proposed here will enable breakthroughs in understanding the structural and molecular basis of fast cellular events including transport, membrane trafficking, cell division, and synaptic transmission.
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The information about "MICROCRYO" are provided by the European Opendata Portal: CORDIS opendata.