Opendata, web and dolomites

IMPACT-HEALTH SIGNED

IMaging Pancreatic Alpha-cells Calcium Tied with HEterogeneous Analysis of Labeled Transcription factors with in situ Hybridization

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

Project "IMPACT-HEALTH" data sheet

The following table provides information about the project.

Coordinator
OSPEDALE SAN RAFFAELE SRL 

Organization address
address: VIA OLGETTINA 60
city: MILANO
postcode: 20132
website: www.hsr.it

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Italy [IT]
 Total cost 168˙277 €
 EC max contribution 168˙277 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2017
 Funding Scheme MSCA-IF-EF-SE
 Starting year 2018
 Duration (year-month-day) from 2018-09-17   to  2020-09-16

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    OSPEDALE SAN RAFFAELE SRL IT (MILANO) coordinator 168˙277.00

Map

 Project objective

Excess of plasma glucagon is frequently reported in diabetic patients, a misregulation that exacerbates hyperglycemia, a major complication of diabetes. This has spurred research into alpha-cell function and glucagon regulation, to find potential treatments, independent from insulin, for diabetes. To normalize glucagon secretion as a therapeutic strategy, it is crucial to have a better understanding of its regulatory mechanisms and the expression of specific genes regulating those mechanisms. Unfortunately, there is no consensus model explaining the regulation of glucagon secretion from pancreatic alpha-cells. However, calcium is known to be required for glucagon secretion, but its role is not completely understood. We propose to evaluate the role of free calcium activity across islet α-cells in the regulation of glucagon secretion from mice, using a custom built light-sheet fluorescence microscope, allowing fast three-dimensional imaging for an extended amount of time. The interest will be focused on the heterogeneity of the response, which forms subpopulations of α-cells. After, the attention will be addressed in discovering cellular expression patterns of transcription factors known to be relevant in alpha-cells through immunofluorescence, and to determine how these regulate their target genes using single molecule Fluorescence In Situ Hybridization (smFISH). Particular attention will be focused on correlating the heterogeneities of calcium activity in subpopulations of alpha-cells with their differential gene expression. By finding this differential expression, it will be possible to target specific genes in order to trigger various single-cells behaviors and discover novel therapeutic targets.

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "IMPACT-HEALTH" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "IMPACT-HEALTH" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.3.2.)

EPIC (2019)

Evolution of Planktonic Gastropod Calcification

Read More  

NeuroSens (2019)

Neuromodulation of Sensory Processing

Read More  

POMOC (2019)

Charles IV and the power of marvellous objects

Read More