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IMaging Pancreatic Alpha-cells Calcium Tied with HEterogeneous Analysis of Labeled Transcription factors with in situ Hybridization

Total Cost €


EC-Contrib. €






Project "IMPACT-HEALTH" data sheet

The following table provides information about the project.


Organization address
address: VIA OLGETTINA 60
city: MILANO
postcode: 20132

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Italy [IT]
 Total cost 168˙277 €
 EC max contribution 168˙277 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2017
 Funding Scheme MSCA-IF-EF-SE
 Starting year 2018
 Duration (year-month-day) from 2018-09-17   to  2020-09-16


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    OSPEDALE SAN RAFFAELE SRL IT (MILANO) coordinator 168˙277.00


 Project objective

Excess of plasma glucagon is frequently reported in diabetic patients, a misregulation that exacerbates hyperglycemia, a major complication of diabetes. This has spurred research into alpha-cell function and glucagon regulation, to find potential treatments, independent from insulin, for diabetes. To normalize glucagon secretion as a therapeutic strategy, it is crucial to have a better understanding of its regulatory mechanisms and the expression of specific genes regulating those mechanisms. Unfortunately, there is no consensus model explaining the regulation of glucagon secretion from pancreatic alpha-cells. However, calcium is known to be required for glucagon secretion, but its role is not completely understood. We propose to evaluate the role of free calcium activity across islet α-cells in the regulation of glucagon secretion from mice, using a custom built light-sheet fluorescence microscope, allowing fast three-dimensional imaging for an extended amount of time. The interest will be focused on the heterogeneity of the response, which forms subpopulations of α-cells. After, the attention will be addressed in discovering cellular expression patterns of transcription factors known to be relevant in alpha-cells through immunofluorescence, and to determine how these regulate their target genes using single molecule Fluorescence In Situ Hybridization (smFISH). Particular attention will be focused on correlating the heterogeneities of calcium activity in subpopulations of alpha-cells with their differential gene expression. By finding this differential expression, it will be possible to target specific genes in order to trigger various single-cells behaviors and discover novel therapeutic targets.

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The information about "IMPACT-HEALTH" are provided by the European Opendata Portal: CORDIS opendata.

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