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HARNESS TERMINATED

Harnessing experimental evolution of rhizobia for an integrative view of endosymbiosis

Total Cost €

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EC-Contrib. €

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Partnership

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 HARNESS project word cloud

Explore the words cloud of the HARNESS project. It provides you a very rough idea of what is the project "HARNESS" about.

host    evolution    time    sequencing    bacteria    plant    survival    biological    frequencies    acquisition    offers    team    prominent    initiated    symbioses    associations    pathogenic    transition    witness    opportunity    chimeric    artificially    shape    infectious    experiment    ralstonia    ecosystems    recapulating    performance    mutualistic    legume    endosymbiotic    functioning    rna    ago    rhizobial    selective    cells    genetics    ing    allelic    altogether    plasmid    countless    populations    plants    agriculture    fixing    beneficial    first    track    extend    analysed    functional    optimise    intracellular    potentially    poorly    fitness    nitrogen    promises    evolutionary    material    progress    combination    solancearum    underpinning    bacterial    leverage    symbiosis    functions    genetic    experimental    pathogen    symbiotic    mutations    eukaryotes    planta    showing    throughput    integrative    accommodation    events    chimera    transcriptional    life    uncover    agro    ancestor    microbiota    transferred    population   

Project "HARNESS" data sheet

The following table provides information about the project.

Coordinator
INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT 

Organization address
address: Rue De L'Universite 147
city: PARIS CEDEX 07
postcode: 75338
website: www.inra.fr

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country France [FR]
 Project website https://www6.toulouse.inra.fr/lipm_eng/Research/Symbiotic-functions-genome-and-evolution-of-rhizobia
 Total cost 196˙707 €
 EC max contribution 196˙707 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2018
 Funding Scheme MSCA-IF-EF-RI
 Starting year 2019
 Duration (year-month-day) from 2019-05-01   to  2021-04-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT FR (PARIS CEDEX 07) coordinator 196˙707.00

Map

 Project objective

Microbiota shape growth and survival of eukaryotes through countless symbiotic associations. A prominent example for agriculture is the mutualistic nitrogen-fixing symbiosis between legume plants and rhizobial bacteria. Understanding the evolution and functioning of these symbioses offers promises to optimise their beneficial use in agro-ecosystems and, potentially, to extend it to non-legume plants. An ambitious project initiated several years ago in the host team aims at recapulating the evolution of new nitrogen-fixing bacteria from a pathogenic ancestor. A symbiotic plasmid was artificially transferred into the plant pathogen Ralstonia solancearum and the resulting chimera was selected for improved in planta symbiotic performance by experimental evolution. This experiment offers a unique opportunity to witness the ‘real-time’ adaptation of chimeric bacteria to their new host plant. In this proposal, I will leverage the biological material generated during this experiment to progress towards an integrative understanding of the evolutionary events underpinning the transition to symbiosis. In particular, I will focus on the acquisition of intracellular uptake and accommodation of bacteria by plant cells, a defining and poorly understood aspect of these symbiotic associations. First, I will use whole-population sequencing to track allelic frequencies in evolving populations and identify mutations improving bacterial intracellular fitness. Functional genetics analyses will then uncover the bacterial functions that are required for endosymbiotic life. Finally, plant transcriptional responses to bacteria showing different infectious abilities will be analysed by RNA-sequencing. Altogether, this work will use a combination of approaches (experimental evolution, genetics and high-throughput sequencing) to advance our understanding of the genetic and selective processes underpinning the evolution of nitrogen-fixing symbioses.

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