Opendata, web and dolomites

RepDiff SIGNED

Revealing novel molecular mechanisms linking DNA replication and cell fate decisions

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 RepDiff project word cloud

Explore the words cloud of the RepDiff project. It provides you a very rough idea of what is the project "RepDiff" about.

ncc    dynamically    significantly    hypothesis    copy    mechanistic    opportunity    inaccessible    determined    accurately    genetic    mechanisms    quantitative    fork    cells    either    fate    works    disruption    stem    chromatin    nascent    bind    undergo    replicate    cell    pluripotent    transcription    decision    affinity    lab    plasticity    regulation    window    replicating    reprogramming    effect    specialized    provides    proteomics    cellular    abnormal    regulators    sequential    all    tool    mass    otherwise    transitions    proteins    link    revealed    join    reveal    time    gene    until    defines    restoration    body    replication    hypothesize    anja    sequence    expertise    organization    silac    share    expert    dna    cancer    profile    entails    predicted    layers    groth    spectrometry    epigenetic    functional    expression    question    discovery    gap    dissect    reconfiguration    purified    edge    preserve    prof    cutting    ahead    post    normal    identity   

Project "RepDiff" data sheet

The following table provides information about the project.

Coordinator
KOBENHAVNS UNIVERSITET 

Organization address
address: NORREGADE 10
city: KOBENHAVN
postcode: 1165
website: www.ku.dk

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Denmark [DK]
 Total cost 219˙312 €
 EC max contribution 219˙312 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2018
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2020
 Duration (year-month-day) from 2020-01-01   to  2021-12-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    KOBENHAVNS UNIVERSITET DK (KOBENHAVN) coordinator 219˙312.00

Map

 Project objective

All cells in our body share the same genetic information. Cellular identity is determined by epigenetic mechanisms, which control gene expression. Replicating cells should accurately replicate their DNA sequence and copy their epigenetic profile to maintain their identity. DNA replication entails the disruption of the chromatin organization ahead of the replication fork and its restoration behind it. When cells change their identity in either normal development or abnormal processes as cancer, they undergo epigenetic reconfiguration, which defines their new identity. Recent works have revealed a time gap between DNA replication and epigenetic state restoration of many chromatin regulation layers. I hypothesize that the time until chromatin restoration post DNA replication provides a ‘window of opportunity’ for transcription factors and chromatin regulators to bind otherwise inaccessible areas and to facilitate chromatin reconfiguration and that pluripotent cells have specialized chromatin replication proteins, which preserve their high epigenetic plasticity. To test this hypothesis, I will join the lab of Prof. Anja Groth, a leading expert in the mechanisms controlling chromatin replication. Together with my expertise in stem cells and reprogramming, I will address this question with two sequential steps. I will use a cutting edge, quantitative proteomics method in which nascent DNA is affinity purified and its associated proteins are analyzed by mass-spectrometry (NCC-SILAC). I will use this discovery tool to define the proteins dynamically associated with nascent chromatin in pluripotent cells and cells that undergo cell fate transitions. I will then investigate proteins predicted to effect chromatin restoration/reconfiguration to dissect their functional role. This work has the potential to reveal a mechanistic link between DNA replication and cell fate decision and thus significantly contribute to the fields of development, stem cells, and cancer.

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "REPDIFF" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "REPDIFF" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.3.2.)

TARGET SLEEP (2020)

Boosting motor learning through sleep and targeted memory reactivation in ageing and Parkinson’s disease

Read More  

Widow Spider Mating (2020)

Immature mating as a novel tactic of an invasive widow spider

Read More  

CP-FTmmW Aminogen (2020)

Chemistry and structure of aminogen radicals using chirped-pulse Fourier transform (sub)millimeter rotational spectroscopy

Read More