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3D-loop SIGNED

Mechanism of homology search and the logic of homologous chromosome pairing in meiosis

Total Cost €

0

EC-Contrib. €

0

Partnership

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 3D-loop project word cloud

Explore the words cloud of the 3D-loop project. It provides you a very rough idea of what is the project "3D-loop" about.

physically    loop    intact    lasting    revealed    joint    donor    repair    synthesis    accurate    lying    chromosome    regulation    harnessed    fundamental    acting    region    mitotic    dynamic    search    basic    achieves    integrates    first    filament    flanking    experimental    cytological    wp1    molecules    vastness    cartography    dsb    uniquely    basis    quantitative    phenomenon    associates    yeast    detect    homolog    assembled    breaks    involvement    overcame    homologs    proximity    nucleoprotein    conserved    regulations    wp2    hurdle    elaborate    strand    efficient    identification    hr    events    light    genome    single    sexual    npf    framework    suite    capacity    sampling    builds    tackle    shed    conundrums    homology    meiosis    core    chromosomal    probe    revisit    re    dna    nucleus    cells    class    crossover    3d    template    homologous    mechanism    cis    paradigm    uses    reproduction    dynamics    ssdna    attachment    engineering    double    pairing    purpose    ligation    molecular    recombination    molecule   

Project "3D-loop" data sheet

The following table provides information about the project.

Coordinator
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS 

Organization address
address: RUE MICHEL ANGE 3
city: PARIS
postcode: 75794
website: www.cnrs.fr

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country France [FR]
 Total cost 1˙499˙779 €
 EC max contribution 1˙499˙779 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2019-STG
 Funding Scheme ERC-STG
 Starting year 2020
 Duration (year-month-day) from 2020-01-01   to  2024-12-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS FR (PARIS) coordinator 1˙499˙779.00

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 Project objective

Homologous recombination (HR) is a conserved DNA double-strand breaks (DSB) repair pathway that uniquely uses an intact DNA molecule as a template. Genome-wide homology search is carried out by a nucleoprotein filament (NPF) assembled on the ssDNA flanking the DSB, and whose product is a “D-loop” joint molecule. Beyond accurate DSB repair, this capacity of HR to spatially associates homologous molecules is also harnessed for homolog pairing in meiosis. The goal of “3D-loop” is to tackle two long lasting conundrums: the fundamental homology search mechanism that achieves accurate and efficient identification of a single homologous donor in the vastness of the genome and nucleus, and how this mechanism is adapted for the purpose of homologs attachment in meiosis. I overcame the main hurdle to study these core steps of HR by developing a suite of proximity ligation-based methodologies and experimental systems to physically detect joint molecules in yeast cells. It revealed elaborate regulation controlling D-loop dynamics and a novel class of joint molecules. This proposal builds upon these methodologies and findings to first address basic properties of the homology sampling process by the NPF and the role of D-loop dynamics, with the long-term goal to establish a quantitative framework of homology search in mitotic cells (WP1). Second, the meiosis-specific regulation of homology search leading to homolog pairing likely integrates chromosomal-scale information. Genome re-synthesis and engineering approaches will be deployed to (i) achieve a quantitative and dynamic cartography of the cytological and molecular events of meiosis over a large chromosomal region, (ii) probe cis-acting regulations at the chromosomal scale, and (iii) revisit the molecular paradigm for crossover formation (WP2). We expect this project to shed light on the fundamental process of homology search and its involvement in the chromosome pairing phenomenon lying at the basis of sexual reproduction.

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The information about "3D-LOOP" are provided by the European Opendata Portal: CORDIS opendata.

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