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3D-loop SIGNED

Mechanism of homology search and the logic of homologous chromosome pairing in meiosis

Total Cost €

0

EC-Contrib. €

0

Partnership

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 3D-loop project word cloud

Explore the words cloud of the 3D-loop project. It provides you a very rough idea of what is the project "3D-loop" about.

identification    dynamics    yeast    intact    chromosomal    probe    regulation    cells    homologous    revealed    donor    cartography    lying    regulations    3d    efficient    accurate    cis    mechanism    suite    framework    nucleoprotein    repair    nucleus    recombination    conundrums    attachment    events    chromosome    core    assembled    uses    template    detect    revisit    crossover    ligation    fundamental    first    search    harnessed    builds    paradigm    filament    engineering    ssdna    vastness    sexual    meiosis    class    elaborate    strand    double    capacity    uniquely    phenomenon    flanking    basis    achieves    loop    joint    wp2    experimental    hr    physically    tackle    integrates    basic    proximity    cytological    molecule    molecules    homology    dsb    conserved    genome    shed    reproduction    molecular    purpose    mitotic    single    homologs    npf    wp1    breaks    involvement    homolog    dna    overcame    hurdle    associates    acting    re    lasting    sampling    region    light    synthesis    quantitative    pairing    dynamic   

Project "3D-loop" data sheet

The following table provides information about the project.

Coordinator
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS 

Organization address
address: RUE MICHEL ANGE 3
city: PARIS
postcode: 75794
website: www.cnrs.fr

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country France [FR]
 Total cost 1˙499˙779 €
 EC max contribution 1˙499˙779 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2019-STG
 Funding Scheme ERC-STG
 Starting year 2020
 Duration (year-month-day) from 2020-01-01   to  2024-12-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS FR (PARIS) coordinator 1˙499˙779.00

Map

 Project objective

Homologous recombination (HR) is a conserved DNA double-strand breaks (DSB) repair pathway that uniquely uses an intact DNA molecule as a template. Genome-wide homology search is carried out by a nucleoprotein filament (NPF) assembled on the ssDNA flanking the DSB, and whose product is a “D-loop” joint molecule. Beyond accurate DSB repair, this capacity of HR to spatially associates homologous molecules is also harnessed for homolog pairing in meiosis. The goal of “3D-loop” is to tackle two long lasting conundrums: the fundamental homology search mechanism that achieves accurate and efficient identification of a single homologous donor in the vastness of the genome and nucleus, and how this mechanism is adapted for the purpose of homologs attachment in meiosis. I overcame the main hurdle to study these core steps of HR by developing a suite of proximity ligation-based methodologies and experimental systems to physically detect joint molecules in yeast cells. It revealed elaborate regulation controlling D-loop dynamics and a novel class of joint molecules. This proposal builds upon these methodologies and findings to first address basic properties of the homology sampling process by the NPF and the role of D-loop dynamics, with the long-term goal to establish a quantitative framework of homology search in mitotic cells (WP1). Second, the meiosis-specific regulation of homology search leading to homolog pairing likely integrates chromosomal-scale information. Genome re-synthesis and engineering approaches will be deployed to (i) achieve a quantitative and dynamic cartography of the cytological and molecular events of meiosis over a large chromosomal region, (ii) probe cis-acting regulations at the chromosomal scale, and (iii) revisit the molecular paradigm for crossover formation (WP2). We expect this project to shed light on the fundamental process of homology search and its involvement in the chromosome pairing phenomenon lying at the basis of sexual reproduction.

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The information about "3D-LOOP" are provided by the European Opendata Portal: CORDIS opendata.

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