NOVO

Novel approaches for prevention and degeneration of pathogenic bacteria biofilms formed on medical devices e.g. catheters

 Coordinatore UNIVERSITAET FUER BODENKULTUR WIEN 

 Organization address address: Gregor Mendel Strasse 33
city: WIEN
postcode: 1180

contact info
Titolo: Prof.
Nome: Georg
Cognome: Guebitz
Email: send email
Telefono: +43 2272 66280 505

 Nazionalità Coordinatore Austria [AT]
 Totale costo 3˙933˙882 €
 EC contributo 2˙971˙045 €
 Programma FP7-HEALTH
Specific Programme "Cooperation": Health
 Code Call FP7-HEALTH-2011-two-stage
 Funding Scheme CP-FP
 Anno di inizio 2012
 Periodo (anno-mese-giorno) 2012-01-01   -   2014-12-31

 Partecipanti

# participant  country  role  EC contrib. [€] 
1    UNIVERSITAET FUER BODENKULTUR WIEN

 Organization address address: Gregor Mendel Strasse 33
city: WIEN
postcode: 1180

contact info
Titolo: Prof.
Nome: Georg
Cognome: Guebitz
Email: send email
Telefono: +43 2272 66280 505

AT (WIEN) coordinator 405˙402.38
2    BAR ILAN UNIVERSITY

 Organization address address: BAR ILAN UNIVERSITY CAMPUS
city: RAMAT GAN
postcode: 52900

contact info
Titolo: Ms.
Nome: Estelle
Cognome: Waise
Email: send email
Telefono: +972 3 5317439
Fax: 97236353277

IL (RAMAT GAN) participant 399˙740.00
3    SYNOVO GMBH

 Organization address address: Paul Ehrlich Str 15
city: TUEBINGEN
postcode: 72076

contact info
Titolo: Mr.
Nome: Michael
Cognome: Burnet
Email: send email
Telefono: +49 178997967672

DE (TUEBINGEN) participant 316˙800.00
4    DI DR ANDREAS PAAR KG

 Organization address address: GIRARDIGASSE 6/1
city: GRAZ
postcode: 8010

contact info
Titolo: Dr.
Nome: Andreas
Cognome: Paar
Email: send email
Telefono: 4369910000000

AT (GRAZ) participant 299˙870.00
5    Associacao Universidade-Empresa para o Desenvolvimento - TecMinho

 Organization address address: Campus de Azurem da Universidade do Minho
city: Guimaraes
postcode: 4800-058

contact info
Titolo: Prof.
Nome: Artur
Cognome: Cavaco
Email: send email
Telefono: +351 93 3263158

PT (Guimaraes) participant 280˙800.00
6    UNIVERSITAET DUISBURG-ESSEN

 Organization address address: UNIVERSITAETSSTRASSE 2
city: ESSEN
postcode: 45141

contact info
Titolo: Ms.
Nome: Kirsten
Cognome: Gercek
Email: send email
Telefono: +49 201 183 2104
Fax: +49 201 183 3265

DE (ESSEN) participant 276˙000.00
7    UNIVERSITAT POLITECNICA DE CATALUNYA

 Organization address address: Jordi Girona 31
city: BARCELONA
postcode: 8034

contact info
Titolo: Ms.
Nome: Cristina
Cognome: Costa
Email: send email
Telefono: +34 93 4017126
Fax: +34 93 4017130

ES (BARCELONA) participant 268˙245.00
8    OSM-DAN LTD.

 Organization address address: Pekeris Street 4
city: REHOVOT
postcode: 76702

contact info
Titolo: Dr.
Nome: Pnina
Cognome: Dan
Email: send email
Telefono: +972 8 9460012
Fax: +972 8 9361486

IL (REHOVOT) participant 219˙410.00
9    DEGANIA SILICONE LTD

 Organization address address: KIBBUTZ DEGANIA BET
city: KIBBUTZ DEGANIA B
postcode: 15130

contact info
Titolo: Mr.
Nome: Oded
Cognome: Stein
Email: send email
Telefono: +972 46755102

IL (KIBBUTZ DEGANIA B) participant 182˙700.00
10    PRONEFRO - PRODUTOS NEFROLOGICOS SA

 Organization address address: RUA B - SECTOR VII LOTE 98
city: MAIA
postcode: 4470-436

contact info
Titolo: Ms.
Nome: Assunção
Cognome: Assunção Mascarenhas
Email: send email
Telefono: +351 229438580

PT (MAIA) participant 164˙240.00
11    MULTIPROFILE HOSPITAL FOR ACTIVE TREATMENT AND EMERGENCY MEDECINE PIROGOV

 Organization address address: TOTLEBEN BLVD 21
city: SOFIA
postcode: 1606

contact info
Titolo: Prof.
Nome: Aneta
Cognome: Hubenova
Email: send email
Telefono: +359 887804206

BG (SOFIA) participant 123˙960.00
12    TECHNISCHE UNIVERSITAET GRAZ

 Organization address address: Rechbauerstrasse 12
city: GRAZ
postcode: 8010

contact info
Titolo: Dr.
Nome: Michaela
Cognome: Pressnig
Email: send email
Telefono: +43 316 873 4319
Fax: +43 316 873 8819

AT (GRAZ) participant 33˙877.62

Mappa


 Word cloud

Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.

nps    nanoparticles    sonochemical    inorganic    bio    pseudomonas    us    mgf    metal    novo    nanocapsules    agent    urinary    inhibition    bacterial    anti    hospital    size    staphylococcus    significantly    medical    successfully    agents    ultrasonic    multidrug    besides    catheters    coatings    ncs    compounds    onto    coating    antibacterial    cdh    acylase    infections    biofilm    biofilms    coated    phenolic    aureus    single    biocompatibility    polymers    resistant    zno    catheter    microbial    proved    antibiofouling    scientists    organic    aeruginosa    amylase    enzymes    quality    cellobiose    oxide    prevent    resistance   

 Obiettivo del progetto (Objective)

'Biofilms are bacterial communities encased in a self-produced hydrated polymeric matrix. An important characteristic of microbial biofilms is their innate resistance to the immune system and susceptibility to antibiotics. This resistance has made microbial biofilms a common cause of medical infections, and difficult-to-treat infections caused by colonized foreign bodies. The NOVO project aims at developing novel approaches to prevent and/or degrade biofilms on catheters elongating their usage in humans up to 10 days. Two complementary approaches for biofilm prophylaxis will be developed: A. Ultrasonic coating of Inorganic antibiofouling agents (process developed by partner BIU) based on a single step sonochemical process to: a) Produce metal fluorides or metal oxides (e.g. MgF2, ZnO) nanoparticles (NPs) and simultaneously b) Impregnate them as antibacterial factors on the catheters. c) Co-coating with bio-inert polymer layers (containing highly hydrophilic antifouling polyethylene glycol, zwitterionic moieties or sugar-groups) grafted onto NPs of adjusted size to the size of MgF2/ZnO NPs or directly onto MgF2/ZnO NPs; to form a hydrogel layer for the protection of the MgF2/ZnO antibiofouling activity. B. Bio/organic antibiofouling activation: 1) Novel coating for catheters based on radical catalyzed polymers to yield anti-bacterial activity. An enzymatic reaction will be applied on the phenolic compounds to generate phenolic radicals to be further polymerized on the catheter surface as an antibiofilm agent. 2) Develop and engineer Cellobiose Dehydrogenases (CDH) that actively oxidizes and degrades biofilms polysaccharides concomitantly producing stoichiometrically H2O2 as antibacterial agent. The enzymes will be coated on the catheters via a lubricant or by the Ultrasonic (US) process after their immobilization. Some novel CDH representatives already show very low activity on glucose which should be removed by further genetic engineering.'

Introduzione (Teaser)

Sonochemical processes prevent biofilms

Descrizione progetto (Article)

Biofilm-contaminated urinary catheters result in annual costs that exceed 400 million US dollars and increase the risk for emergence of multidrug-resistant bacteria. Pseudomonas aeruginosa and Staphylococcus aureus are the most common microbes in hospital settings that are multidrug resistant.

Different approaches have been attempted to prevent biofilm formation that include biological mechanisms and impregnation of medical devices with anti-microbial agents but they proved inefficient.

The EU-funded http://www.fp7-novo.eu (NOVO) project has exploited the low-cost, single-step, eco-friendly ultrasound (US) for inhibition of biofilm formation to produce novel anti-microbial coatings and materials. For coatings, researchers explored the efficacy of inorganic nanoparticles (NPs) like zinc oxide and magnesium fluoride (MgF2) as well as organic polymers and enzymes.

Scientists successfully coated inorganic particles onto catheters with MgF2 showing over 70% inhibition of biofilm formation. The entire sonochemical process for catheter coating took less than 30 minutes. Besides retaining biocompatibility, the catheter coatings were unaffected by sterilisation with gamma-radiation and ethylene oxide. Importantly, the coated catheter properties complied with regulatory requirements.

Coating of silicone catheters with organic polymers successfully reduced Pseudomonas aeruginosa and Staphylococcus aureus bacterial biofilm formation by over 50%. Modification conditions for the organic solvents were also optimised.

Coating catheters with enzymatically polymerised phenolic compounds yielded excellent results. Tests demonstrated over 80% anti-microbial activity in several bacterial species. Besides this, phenolic nanocapsules (e.g. epigallocatechin gallate nanocapsules (EGCG NCs)) were also coated onto catheters under varying conditions to optimise performance. Use of sulfobetaine methacrylate (SBMA) with phenolic NCs significantly improved biofilm inhibition.

In parallel, scientists immobilised enzymes like cellobiose dehydrogenase (CDH), amylase and acylase on catheter surfaces. CDH, acylase and amylase coatings also proved effective in reducing P. aeruginosa and S. aureus biofilm formations. Future work will focus on evaluating and optimising their anti-biofilm efficiency, toxicity, biocompatibility and longevity.

Commercialisation of NOVO products and techniques for coating medical devices such as implants and urinary catheters promises to cost-effectively and significantly reduce hospital-acquired infections. Besides improving healthcare quality and patients' quality of life, this will also boost the job market as well as European economy.

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