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FLYghtCaRe

Ca2+ feedback control of TRP/TRPL channels in Drosophila photoreceptors

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EC-Contrib. €

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 FLYghtCaRe project word cloud

Explore the words cloud of the FLYghtCaRe project. It provides you a very rough idea of what is the project "FLYghtCaRe" about.

ambient    dissecting    rods    sophistication    dark    genetically    world    activated    phototransduction    hypotheses    core    eye    laboratory    ca2    visual    enriching    rising    electrophysiology    positive    fruitfly    calmodulin    displaying    indicators    compound    binding    encoded    night    mutants    degeneration    bump    channels    first    fly    bumps    physiological    optogenetics    photoreceptors    midday    sun    difference    phenotypes    precise    phototransductive    expressing    resolution    class    made    professional    dynamics    optics    daylight    termination    amplification    subcellular    ciliary    detect    imaged    intact    lie    cell    modulates    illumination    regulatory    cellular    light    virtually    chimeric    interaction    obtain    responsible    mechanisms    generating    receptor    starlit    characterise    transient    feedback    continue    performance    gain    flies    combine    trpl    mutated    rhabdomeric    spatial    entry    responding    sites    single    full    retinal    defective    cascade    themselves    trp    photons    dramatic    vertebrates    molecular    will    compartments    quantum    contrast    skills    negative    signalling    reliably    loops    explore    unknown   

Project "FLYghtCaRe" data sheet

The following table provides information about the project.

Coordinator
THE CHANCELLOR MASTERS AND SCHOLARSOF THE UNIVERSITY OF CAMBRIDGE 

Organization address
address: TRINITY LANE THE OLD SCHOOLS
city: CAMBRIDGE
postcode: CB2 1TN
website: www.cam.ac.uk

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Project website https://www.pdn.cam.ac.uk/directory/asteriti
 Total cost 183˙454 €
 EC max contribution 183˙454 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2014
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2015
 Duration (year-month-day) from 2015-11-01   to  2017-10-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    THE CHANCELLOR MASTERS AND SCHOLARSOF THE UNIVERSITY OF CAMBRIDGE UK (CAMBRIDGE) coordinator 183˙454.00

Map

 Project objective

A major challenge for any visual system is the dramatic difference in ambient light between a starlit night and midday sun. In contrast to the ciliary rods of vertebrates, the rhabdomeric photoreceptors of the common fruitfly not only reliably detect single photons when dark-adapted (generating responses called ‘quantum bumps’), but continue responding in full daylight. At the core of this performance lie positive and negative feedback loops, many involving Ca2 entry through the light activated transient receptor potential channels TRP and TRPL. Although Ca2 modulates virtually every step of the phototransductive cascade, key targets are the channels themselves, leading to: (i) amplification by positive feedback during the rising phase of quantum bumps, (ii) gain reduction by negative feedback during quantum bump termination and during light adaptation. Our objectives are to combine single cell electrophysiology and optogenetics to explore Ca2 signalling in fly photoreceptors, dissecting both its molecular interaction with TRP and TRPL channels and its dynamics within the cell. Our first aim will be to characterise the role of Calmodulin binding sites and other unknown regulatory sites on TRP and TRPL, in the feedback loops described above. Flies expressing mutated and chimeric TRP/TRPL channels will be used. Our second aim will be to obtain measurements of Ca2 dynamics with subcellular spatial resolution under physiological illumination. Genetically encoded Ca2 indicators will be targeted to specific cellular compartments and imaged in intact flies by exploiting the optics of the compound eye. Measurements will be made in fly mutants displaying defective light responses and retinal degeneration, leading to precise hypotheses on the mechanisms responsible for their phenotypes. This project will bring our understanding of rhabdomeric phototransduction to a new level of sophistication, while enriching my professional skills in a world-class laboratory.

 Publications

year authors and title journal last update
List of publications.
2017 Sabrina Asteriti, Che-Hsiung Liu, Roger C. Hardie
Calcium signalling in Drosophila photoreceptors measured with GCaMP6f
published pages: , ISSN: 0143-4160, DOI: 10.1016/j.ceca.2017.02.006
Cell Calcium 2019-06-14

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