Explore the words cloud of the CellStructure project. It provides you a very rough idea of what is the project "CellStructure" about.
The following table provides information about the project.
EUROPEAN MOLECULAR BIOLOGY LABORATORY
|Coordinator Country||Germany [DE]|
|Total cost||1˙686˙469 €|
|EC max contribution||1˙686˙469 € (100%)|
1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
|Duration (year-month-day)||from 2017-06-01 to 2022-05-31|
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|1||EUROPEAN MOLECULAR BIOLOGY LABORATORY||DE (HEIDELBERG)||coordinator||1˙686˙469.00|
Supra-molecular protein machineries control diverse cellular processes. Knowing their structural organization is crucial for understanding their function. As classical structural biology techniques are limited in studying such assemblies in their natural cellular environment, there is a critical methodological gap inhibiting a direct link between structure and function. Consequently, the structural intermediates underlying a full activity cycle of a large multi-protein complex have been impossible to visualize. Recent advances in fluorescence microscopy, in particular the development of groundbreaking superresolution microscopy (SRM) methods, can now help bridge this gap. With this interdisciplinary proposal, my group will develop unique and innovative optical, biological and computational imaging technologies to determine the structural organization of multi-protein assemblies in their functional cellular context. We will reach this goal by developing a method to robustly measure the precise 3D arrangements of proteins in supra-molecular assemblies in situ with nanometer isotropic resolution based on supercritical-angle detection and by measuring their absolute stoichiometries with engineered counting standards. We will also develop new data analysis tools to statistically analyze such data, taking into account the functional cellular context measured with correlative superresolution and electron microscopy, multi-color SRM and molecular biology tools. We will apply these new methods to address key questions on endocytosis, a fundamental membrane trafficking process. Our aim is to determine a time-resolved 3D superresolution localization map of the yeast endocytic proteins during the major functional transitions and to integrate these data into a mechanistic model of endocytosis. Importantly, the methods we develop here can be applied to many other large protein-based machines, and thus have the potential to have high impact in other key areas of cell biology.
|year||authors and title||journal||last update|
Daniel SchrÃ¶der, Joran Deschamps, Anindita Dasgupta, Ulf Matti, Jonas Ries
Cost-efficient open source laser engine for microscopy
published pages: 609, ISSN: 2156-7085, DOI: 10.1364/boe.380815
|Biomedical Optics Express 11/2||2020-02-05|
Rieger, B., Stallinga, S., Heydarian, H., Schueder, F., Jungmann, R., Ries, J., Przybylski, A., Bates, M., Keller-Findeisen, J., and van Werkhoven, B.
Three dimensional particle averaging for structural imaging of macromolecular complexes by localization microscopy
published pages: , ISSN: , DOI: 10.1101/837575
Michelle S. Frei, Philipp Hoess, Marko Lampe, Bianca Nijmeijer, Moritz Kueblbeck, Jan Ellenberg, Hubert Wadepohl, Jonas Ries, Stefan Pitsch, Luc Reymond, Kai Johnsson
Photoactivation of silicon rhodamines via a light-induced protonation
published pages: , ISSN: 2041-1723, DOI: 10.1038/s41467-019-12480-3
|Nature Communications 10/1||2020-02-05|
Thomas Schlichthaerle, Maximilian T. Strauss, Florian Schueder, Alexander Auer, Bianca Nijmeijer, Moritz Kueblbeck, Vilma Jimenezâ€…Sabinina, Jervis V. Thevathasan, Jonas Ries, Jan Ellenberg, Ralf Jungmann
Direct Visualization of Single Nuclear Pore Complex Proteins Using Genetically-Encoded Probes for DNA-PAINT
published pages: 13004-13008, ISSN: 1433-7851, DOI: 10.1002/anie.201905685
|Angewandte Chemie International Edition 58/37||2020-02-05|
Jervis Vermal Thevathasan, Maurice Kahnwald, Konstanty CieÅ›liÅ„ski, Philipp Hoess, Sudheer Kumar Peneti, Manuel Reitberger, Daniel Heid, Krishna Chaitanya Kasuba, Sarah Janice Hoerner, Yiming Li, Yu-Le Wu, Markus Mund, Ulf Matti, Pedro Matos Pereira, Ricardo Henriques, Bianca Nijmeijer, Moritz Kueblbeck, Vilma Jimenez Sabinina, Jan Ellenberg, Jonas Ries
Nuclear pores as versatile reference standards for quantitative superresolution microscopy
published pages: 1045-1053, ISSN: 1548-7091, DOI: 10.1038/s41592-019-0574-9
|Nature Methods 16/10||2020-02-05|
Yiming Li, Yu-Le Wu, Philipp Hoess, Markus Mund, Jonas Ries
Depth-dependent PSF calibration and aberration correction for 3D single-molecule localization
published pages: 2708, ISSN: 2156-7085, DOI: 10.1364/boe.10.002708
|Biomedical Optics Express 10/6||2020-02-05|
Daniel Sage, Thanh-An Pham, Hazen Babcock, Tomas Lukes, Thomas Pengo, Jerry Chao, Ramraj Velmurugan, Alex Herbert, Anurag Agrawal, Silvia Colabrese, Ann Wheeler, Anna Archetti, Bernd Rieger, Raimund Ober, Guy M. Hagen, Jean-Baptiste Sibarita, Jonas Ries, Ricardo Henriques, Michael Unser, Seamus Holden
Super-resolution fight club: assessment of 2D and 3D single-molecule localization microscopy software
published pages: 387-395, ISSN: 1548-7091, DOI: 10.1038/s41592-019-0364-4
|Nature Methods 16/5||2020-02-05|
Klaus C. Gwosch, Jasmin K. Pape, Francisco Balzarotti, Philipp Hoess, Jan Ellenberg, Jonas Ries, Stefan W. Hell
MINFLUX nanoscopy delivers 3D multicolor nanometer resolution in cells
published pages: , ISSN: 1548-7091, DOI: 10.1038/s41592-019-0688-0
Markus Mund, Johannes Albertus van der Beek, Joran Deschamps, Serge Dmitrieff, Philipp Hoess, Jooske Louise Monster, Andrea Picco, FranÃ§ois NÃ©dÃ©lec, Marko Kaksonen, Jonas Ries
Systematic Nanoscale Analysis of Endocytosis Links Efficient Vesicle Formation to Patterned Actin Nucleation
published pages: 884-896.e17, ISSN: 0092-8674, DOI: 10.1016/j.cell.2018.06.032
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