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CellStructure SIGNED

Structural cell biology in situ using superresolution microscopy

Total Cost €

0

EC-Contrib. €

0

Partnership

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 CellStructure project word cloud

Explore the words cloud of the CellStructure project. It provides you a very rough idea of what is the project "CellStructure" about.

knowing    proteins    group    standards    questions    measuring    innovative    critical    trafficking    localization    structure    analyze    arrangements    intermediates    inhibiting    bridge    cellular    function    assemblies    model    resolution    isotropic    correlative    technologies    endocytosis    machines    cycle    electron    cell    color    counting    biological    visualize    machineries    optical    endocytic    engineered    integrate    microscopy    diverse    structural    transitions    supercritical    yeast    precise    tools    biology    functional    environment    detection    organization    stoichiometries    underlying    map    angle    techniques    3d    robustly    gap    srm    superresolution    methodological    consequently    supra    time    molecular    resolved    context    measured    fluorescence    groundbreaking    limited    direct    nanometer    mechanistic    link    computational    absolute    full    data    fundamental    imaging    impossible    natural    protein    membrane    situ    statistically    interdisciplinary   

Project "CellStructure" data sheet

The following table provides information about the project.

Coordinator
EUROPEAN MOLECULAR BIOLOGY LABORATORY 

Organization address
address: Meyerhofstrasse 1
city: HEIDELBERG
postcode: 69117
website: http://www.embl.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Project website https://intranet.embl.de/research/cbb/ries/index.html
 Total cost 1˙686˙469 €
 EC max contribution 1˙686˙469 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2016-COG
 Funding Scheme ERC-COG
 Starting year 2017
 Duration (year-month-day) from 2017-06-01   to  2022-05-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    EUROPEAN MOLECULAR BIOLOGY LABORATORY DE (HEIDELBERG) coordinator 1˙686˙469.00

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 Project objective

Supra-molecular protein machineries control diverse cellular processes. Knowing their structural organization is crucial for understanding their function. As classical structural biology techniques are limited in studying such assemblies in their natural cellular environment, there is a critical methodological gap inhibiting a direct link between structure and function. Consequently, the structural intermediates underlying a full activity cycle of a large multi-protein complex have been impossible to visualize. Recent advances in fluorescence microscopy, in particular the development of groundbreaking superresolution microscopy (SRM) methods, can now help bridge this gap. With this interdisciplinary proposal, my group will develop unique and innovative optical, biological and computational imaging technologies to determine the structural organization of multi-protein assemblies in their functional cellular context. We will reach this goal by developing a method to robustly measure the precise 3D arrangements of proteins in supra-molecular assemblies in situ with nanometer isotropic resolution based on supercritical-angle detection and by measuring their absolute stoichiometries with engineered counting standards. We will also develop new data analysis tools to statistically analyze such data, taking into account the functional cellular context measured with correlative superresolution and electron microscopy, multi-color SRM and molecular biology tools. We will apply these new methods to address key questions on endocytosis, a fundamental membrane trafficking process. Our aim is to determine a time-resolved 3D superresolution localization map of the yeast endocytic proteins during the major functional transitions and to integrate these data into a mechanistic model of endocytosis. Importantly, the methods we develop here can be applied to many other large protein-based machines, and thus have the potential to have high impact in other key areas of cell biology.

 Publications

year authors and title journal last update
List of publications.
2020 Daniel Schröder, Joran Deschamps, Anindita Dasgupta, Ulf Matti, Jonas Ries
Cost-efficient open source laser engine for microscopy
published pages: 609, ISSN: 2156-7085, DOI: 10.1364/boe.380815
Biomedical Optics Express 11/2 2020-02-05
2019 Rieger, B., Stallinga, S., Heydarian, H., Schueder, F., Jungmann, R., Ries, J., Przybylski, A., Bates, M., Keller-Findeisen, J., and van Werkhoven, B.
Three dimensional particle averaging for structural imaging of macromolecular complexes by localization microscopy
published pages: , ISSN: , DOI: 10.1101/837575
bioRxiv 2020-02-05
2019 Michelle S. Frei, Philipp Hoess, Marko Lampe, Bianca Nijmeijer, Moritz Kueblbeck, Jan Ellenberg, Hubert Wadepohl, Jonas Ries, Stefan Pitsch, Luc Reymond, Kai Johnsson
Photoactivation of silicon rhodamines via a light-induced protonation
published pages: , ISSN: 2041-1723, DOI: 10.1038/s41467-019-12480-3
Nature Communications 10/1 2020-02-05
2019 Thomas Schlichthaerle, Maximilian T. Strauss, Florian Schueder, Alexander Auer, Bianca Nijmeijer, Moritz Kueblbeck, Vilma Jimenez Sabinina, Jervis V. Thevathasan, Jonas Ries, Jan Ellenberg, Ralf Jungmann
Direct Visualization of Single Nuclear Pore Complex Proteins Using Genetically-Encoded Probes for DNA-PAINT
published pages: 13004-13008, ISSN: 1433-7851, DOI: 10.1002/anie.201905685
Angewandte Chemie International Edition 58/37 2020-02-05
2019 Jervis Vermal Thevathasan, Maurice Kahnwald, Konstanty Cieśliński, Philipp Hoess, Sudheer Kumar Peneti, Manuel Reitberger, Daniel Heid, Krishna Chaitanya Kasuba, Sarah Janice Hoerner, Yiming Li, Yu-Le Wu, Markus Mund, Ulf Matti, Pedro Matos Pereira, Ricardo Henriques, Bianca Nijmeijer, Moritz Kueblbeck, Vilma Jimenez Sabinina, Jan Ellenberg, Jonas Ries
Nuclear pores as versatile reference standards for quantitative superresolution microscopy
published pages: 1045-1053, ISSN: 1548-7091, DOI: 10.1038/s41592-019-0574-9
Nature Methods 16/10 2020-02-05
2019 Yiming Li, Yu-Le Wu, Philipp Hoess, Markus Mund, Jonas Ries
Depth-dependent PSF calibration and aberration correction for 3D single-molecule localization
published pages: 2708, ISSN: 2156-7085, DOI: 10.1364/boe.10.002708
Biomedical Optics Express 10/6 2020-02-05
2019 Daniel Sage, Thanh-An Pham, Hazen Babcock, Tomas Lukes, Thomas Pengo, Jerry Chao, Ramraj Velmurugan, Alex Herbert, Anurag Agrawal, Silvia Colabrese, Ann Wheeler, Anna Archetti, Bernd Rieger, Raimund Ober, Guy M. Hagen, Jean-Baptiste Sibarita, Jonas Ries, Ricardo Henriques, Michael Unser, Seamus Holden
Super-resolution fight club: assessment of 2D and 3D single-molecule localization microscopy software
published pages: 387-395, ISSN: 1548-7091, DOI: 10.1038/s41592-019-0364-4
Nature Methods 16/5 2020-02-05
2020 Klaus C. Gwosch, Jasmin K. Pape, Francisco Balzarotti, Philipp Hoess, Jan Ellenberg, Jonas Ries, Stefan W. Hell
MINFLUX nanoscopy delivers 3D multicolor nanometer resolution in cells
published pages: , ISSN: 1548-7091, DOI: 10.1038/s41592-019-0688-0
Nature Methods 2020-02-05
2018 Markus Mund, Johannes Albertus van der Beek, Joran Deschamps, Serge Dmitrieff, Philipp Hoess, Jooske Louise Monster, Andrea Picco, François Nédélec, Marko Kaksonen, Jonas Ries
Systematic Nanoscale Analysis of Endocytosis Links Efficient Vesicle Formation to Patterned Actin Nucleation
published pages: 884-896.e17, ISSN: 0092-8674, DOI: 10.1016/j.cell.2018.06.032
Cell 174/4 2019-10-08

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The information about "CELLSTRUCTURE" are provided by the European Opendata Portal: CORDIS opendata.

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