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CellStructure SIGNED

Structural cell biology in situ using superresolution microscopy

Total Cost €


EC-Contrib. €






 CellStructure project word cloud

Explore the words cloud of the CellStructure project. It provides you a very rough idea of what is the project "CellStructure" about.

stoichiometries    robustly    srm    standards    technologies    imaging    counting    questions    methodological    analyze    machineries    intermediates    membrane    natural    context    measured    full    fundamental    detection    group    trafficking    mechanistic    assemblies    knowing    innovative    structure    machines    protein    statistically    computational    absolute    supercritical    model    yeast    optical    fluorescence    measuring    structural    molecular    cellular    inhibiting    color    limited    biology    function    gap    situ    precise    integrate    biological    cell    direct    underlying    engineered    visualize    organization    consequently    functional    correlative    3d    bridge    cycle    supra    transitions    groundbreaking    resolved    data    diverse    superresolution    techniques    impossible    isotropic    proteins    environment    arrangements    map    localization    endocytic    endocytosis    microscopy    interdisciplinary    critical    resolution    link    angle    nanometer    tools    time    electron   

Project "CellStructure" data sheet

The following table provides information about the project.


Organization address
address: Meyerhofstrasse 1
postcode: 69117

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Project website
 Total cost 1˙686˙469 €
 EC max contribution 1˙686˙469 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2016-COG
 Funding Scheme ERC-COG
 Starting year 2017
 Duration (year-month-day) from 2017-06-01   to  2022-05-31


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 


 Project objective

Supra-molecular protein machineries control diverse cellular processes. Knowing their structural organization is crucial for understanding their function. As classical structural biology techniques are limited in studying such assemblies in their natural cellular environment, there is a critical methodological gap inhibiting a direct link between structure and function. Consequently, the structural intermediates underlying a full activity cycle of a large multi-protein complex have been impossible to visualize. Recent advances in fluorescence microscopy, in particular the development of groundbreaking superresolution microscopy (SRM) methods, can now help bridge this gap. With this interdisciplinary proposal, my group will develop unique and innovative optical, biological and computational imaging technologies to determine the structural organization of multi-protein assemblies in their functional cellular context. We will reach this goal by developing a method to robustly measure the precise 3D arrangements of proteins in supra-molecular assemblies in situ with nanometer isotropic resolution based on supercritical-angle detection and by measuring their absolute stoichiometries with engineered counting standards. We will also develop new data analysis tools to statistically analyze such data, taking into account the functional cellular context measured with correlative superresolution and electron microscopy, multi-color SRM and molecular biology tools. We will apply these new methods to address key questions on endocytosis, a fundamental membrane trafficking process. Our aim is to determine a time-resolved 3D superresolution localization map of the yeast endocytic proteins during the major functional transitions and to integrate these data into a mechanistic model of endocytosis. Importantly, the methods we develop here can be applied to many other large protein-based machines, and thus have the potential to have high impact in other key areas of cell biology.


year authors and title journal last update
List of publications.
2020 Daniel Schröder, Joran Deschamps, Anindita Dasgupta, Ulf Matti, Jonas Ries
Cost-efficient open source laser engine for microscopy
published pages: 609, ISSN: 2156-7085, DOI: 10.1364/boe.380815
Biomedical Optics Express 11/2 2020-02-05
2019 Rieger, B., Stallinga, S., Heydarian, H., Schueder, F., Jungmann, R., Ries, J., Przybylski, A., Bates, M., Keller-Findeisen, J., and van Werkhoven, B.
Three dimensional particle averaging for structural imaging of macromolecular complexes by localization microscopy
published pages: , ISSN: , DOI: 10.1101/837575
bioRxiv 2020-02-05
2019 Michelle S. Frei, Philipp Hoess, Marko Lampe, Bianca Nijmeijer, Moritz Kueblbeck, Jan Ellenberg, Hubert Wadepohl, Jonas Ries, Stefan Pitsch, Luc Reymond, Kai Johnsson
Photoactivation of silicon rhodamines via a light-induced protonation
published pages: , ISSN: 2041-1723, DOI: 10.1038/s41467-019-12480-3
Nature Communications 10/1 2020-02-05
2019 Thomas Schlichthaerle, Maximilian T. Strauss, Florian Schueder, Alexander Auer, Bianca Nijmeijer, Moritz Kueblbeck, Vilma Jimenez Sabinina, Jervis V. Thevathasan, Jonas Ries, Jan Ellenberg, Ralf Jungmann
Direct Visualization of Single Nuclear Pore Complex Proteins Using Genetically-Encoded Probes for DNA-PAINT
published pages: 13004-13008, ISSN: 1433-7851, DOI: 10.1002/anie.201905685
Angewandte Chemie International Edition 58/37 2020-02-05
2019 Jervis Vermal Thevathasan, Maurice Kahnwald, Konstanty Cieśliński, Philipp Hoess, Sudheer Kumar Peneti, Manuel Reitberger, Daniel Heid, Krishna Chaitanya Kasuba, Sarah Janice Hoerner, Yiming Li, Yu-Le Wu, Markus Mund, Ulf Matti, Pedro Matos Pereira, Ricardo Henriques, Bianca Nijmeijer, Moritz Kueblbeck, Vilma Jimenez Sabinina, Jan Ellenberg, Jonas Ries
Nuclear pores as versatile reference standards for quantitative superresolution microscopy
published pages: 1045-1053, ISSN: 1548-7091, DOI: 10.1038/s41592-019-0574-9
Nature Methods 16/10 2020-02-05
2019 Yiming Li, Yu-Le Wu, Philipp Hoess, Markus Mund, Jonas Ries
Depth-dependent PSF calibration and aberration correction for 3D single-molecule localization
published pages: 2708, ISSN: 2156-7085, DOI: 10.1364/boe.10.002708
Biomedical Optics Express 10/6 2020-02-05
2019 Daniel Sage, Thanh-An Pham, Hazen Babcock, Tomas Lukes, Thomas Pengo, Jerry Chao, Ramraj Velmurugan, Alex Herbert, Anurag Agrawal, Silvia Colabrese, Ann Wheeler, Anna Archetti, Bernd Rieger, Raimund Ober, Guy M. Hagen, Jean-Baptiste Sibarita, Jonas Ries, Ricardo Henriques, Michael Unser, Seamus Holden
Super-resolution fight club: assessment of 2D and 3D single-molecule localization microscopy software
published pages: 387-395, ISSN: 1548-7091, DOI: 10.1038/s41592-019-0364-4
Nature Methods 16/5 2020-02-05
2020 Klaus C. Gwosch, Jasmin K. Pape, Francisco Balzarotti, Philipp Hoess, Jan Ellenberg, Jonas Ries, Stefan W. Hell
MINFLUX nanoscopy delivers 3D multicolor nanometer resolution in cells
published pages: , ISSN: 1548-7091, DOI: 10.1038/s41592-019-0688-0
Nature Methods 2020-02-05
2018 Markus Mund, Johannes Albertus van der Beek, Joran Deschamps, Serge Dmitrieff, Philipp Hoess, Jooske Louise Monster, Andrea Picco, François Nédélec, Marko Kaksonen, Jonas Ries
Systematic Nanoscale Analysis of Endocytosis Links Efficient Vesicle Formation to Patterned Actin Nucleation
published pages: 884-896.e17, ISSN: 0092-8674, DOI: 10.1016/j.cell.2018.06.032
Cell 174/4 2019-10-08

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