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GeneREFORM SIGNED

Genetically Encoded Multicolor Reporter Systems For Multiplexed MRI

Total Cost €

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EC-Contrib. €

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Partnership

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 GeneREFORM project word cloud

Explore the words cloud of the GeneREFORM project. It provides you a very rough idea of what is the project "GeneREFORM" about.

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Project "GeneREFORM" data sheet

The following table provides information about the project.

Coordinator
WEIZMANN INSTITUTE OF SCIENCE 

Organization address
address: HERZL STREET 234
city: REHOVOT
postcode: 7610001
website: www.weizmann.ac.il

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Israel [IL]
 Total cost 1˙478˙284 €
 EC max contribution 1˙478˙284 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2015-STG
 Funding Scheme ERC-STG
 Starting year 2016
 Duration (year-month-day) from 2016-05-01   to  2021-04-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    WEIZMANN INSTITUTE OF SCIENCE IL (REHOVOT) coordinator 1˙478˙284.00

Map

 Project objective

In order to fully understand the complexity of biological processes that are reflected by simultaneous occurrences of intra and inter-cellular events, multiplexed imaging platforms are needed. Fluorescent reporter genes, with their “multicolor” imaging capabilities, have revolutionized science and their founders have been awarded the Nobel Prize. Nevertheless, the light signal source of these reporters, which restricts their use in deep tissues and in large animals (and potentially in humans), calls for alternatives. Reporter genes for MRI, although in their infancy, showed several exceptionalities, including the ability to longitudinal study the same subject with unlimited tissue penetration and to coregister information from reporter gene expression with high-resolution anatomical images. Inspired by the multicolor capabilities of optical reporter genes, this proposal aims to develop, optimize, and implement genetically engineered reporter systems for MRI with artificial “multicolor” characteristics. Capitalizing on (i) the Chemical Exchange Saturation Transfer (CEST)-MRI contrast mechanism that allows the use of small bioorganic molecules as MRI sensors, (ii) the frequency encoding, color-like features of CEST, and on (iii) enzyme engineering procedures that allow the optimization of enzymatic activity for a desired substrate, a “multicolor” genetically encoded MRI reporter system is proposed. By (a) synthesizing libraries of non-natural nucleosides (“reporter probes”) to generate artificially “colored” CEST contrast, and (b) performing directed evolution of deoxyribonucleoside kinase (dNK) enzymes (“reporter genes”) to phosphorylate those nucleosides, the “multicolor” genetically encoded MRI “reporter system” will be created. The orthogonally of the obtained pairs of substrate (CEST sensor)/ enzyme (mutant dNK) will allow their simultaneous use as a genetically encoded reporter system for in vivo “multicolor” monitoring of reporter gene expression with MRI.

 Publications

year authors and title journal last update
List of publications.
2018 Idan Ashur, Hyla Allouche-Arnon, Amnon Bar-Shir
Calcium Fluoride Nanocrystals: Tracers for In Vivo 19 F Magnetic Resonance Imaging
published pages: 7478-7482, ISSN: 1433-7851, DOI: 10.1002/anie.201800838
Angewandte Chemie International Edition 57/25 2019-10-29

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