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ChromaSTORM SIGNED

Visualising how proteins fold DNA into topologically associating domains in single human cells

Total Cost €

0

EC-Contrib. €

0

Partnership

0

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 ChromaSTORM project word cloud

Explore the words cloud of the ChromaSTORM project. It provides you a very rough idea of what is the project "ChromaSTORM" about.

vital    structures    structuring    microscopy    imaging    additionally    emerge    mediator    core    backbone    copy    proximity    tools    ligation    quantitative    function    advent    cohesin    manner    building    revealed    domains    genomic    variations    situ    simultaneously    chromatin    nm    modulated    transcription    reveal    enigmatic    protein    despite    fundamental    corresponds    employ    proteins    information    how    spatial    cell    context    multidisciplinary    individual    cells    functional    integrating    data    last    tad    3d    human    largely    absolute    derive    resolution    principles    nucleosome    dynamics    repair    organisation    tads    blocks    organisers    genetic    condensin    prerequisite    depletion    inside    regulates    topologically    carry    genome    first    visualise    associating    functions    nucleosomes    architectural    hierarchical    ctcf    few    replication    structure    super    understand    architecture    description    phenotypic    decade    dna    acute    model    inner    size   

Project "ChromaSTORM" data sheet

The following table provides information about the project.

Coordinator
EUROPEAN MOLECULAR BIOLOGY LABORATORY 

Organization address
address: Meyerhofstrasse 1
city: HEIDELBERG
postcode: 69117
website: http://www.embl.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 174˙806 €
 EC max contribution 174˙806 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2018
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2020
 Duration (year-month-day) from 2020-09-01   to  2022-08-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    EUROPEAN MOLECULAR BIOLOGY LABORATORY DE (HEIDELBERG) coordinator 174˙806.00

Map

 Project objective

How can phenotypic variations emerge from cells that carry the same genetic information? It is one of today’s great challenges to understand how genetic information is modulated inside the cell. Over the last decade, insight from genome-wide proximity-based ligation approaches revealed that the genome is organised in a hierarchical manner with the help of structuring proteins, and that this spatial organisation regulates the core functions of the genome, such as transcription, replication and repair. In this context, topologically associating domains (TADs) were identified as fundamental and functional building blocks of chromatin organisation above the nucleosome level. Despite its vital importance, our current understanding of the spatial organisation of TADs remains largely enigmatic. With the advent of super-resolution microscopy, tools are now available for studying genomic structures and their functional dynamics in situ at a resolution of 10 nm which corresponds to the size of a few nucleosomes. The goal of this project is to reveal the principles of TAD organisation in human cells. To achieve this, I will employ a multidisciplinary imaging-based approach. I will simultaneously visualise the DNA backbone of TADs and architectural proteins involved in TAD structure applying 3D super-resolution microscopy. I will focus on the key TAD organisers CTCF and Cohesin, as well as on Mediator and Condensin II. Additionally, I will directly study their individual structuring function for TADs by their acute depletion. Integrating these data with quantitative measurements of absolute protein copy numbers, I will derive a data-driven model of inner TAD organisation in cells. These studies will provide the first 3D description of this fundamental chromatin super-structure and will further our understanding of genome architecture which is a prerequisite for understanding genome function.

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The information about "CHROMASTORM" are provided by the European Opendata Portal: CORDIS opendata.

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lastchecktime (2020-12-01 12:00:27) correctly updated