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IntestineUb

The role of Ubiquitin System on the homeostatic control of stem cell maintenance and differentiation in crypt stem cells

Total Cost €

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EC-Contrib. €

0

Partnership

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 IntestineUb project word cloud

Explore the words cloud of the IntestineUb project. It provides you a very rough idea of what is the project "IntestineUb" about.

arrayed    localization    tight    recapitulates    surface    primary    advantage    fuelled    self    biological    e3    tissue    intestinal    renewal    assay    components    mis    biochemical    maintenance    translational    molecule    differentiation    inhibitors    cas9    proteins    modifications    cell    mammalian    epithelium    cellular    essentially    screening    mechanism    bioid    ubiquitination    vitro    small    generation    amenable    either    cells    adult    knockout    technologies    hyperplasia    near    performing    organoids    tissues    adequately    crypt    reside    organization    population    effect    powerful    experimental    multipotency    modification    models    optimal    phenotype    homeostatic    signalling    sized    intestine    regulation    caused    crispr    pattern    serves    transcriptional    culture    attenuate    conditional    mouse    function    ligases    proteome    mutation    post    ip    vivo    conducting    candidate    characterization    extensively    organoid    sub    rate    sustain    little    elucidate    lines    expression    stem    model    homeostasis    gene    mass    regarding    ubiquitin    reporter   

Project "IntestineUb" data sheet

The following table provides information about the project.

Coordinator
THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE 

Organization address
address: TRINITY LANE THE OLD SCHOOLS
city: CAMBRIDGE
postcode: CB2 1TN
website: www.cam.ac.uk

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Project website https://www.imba.oeaw.ac.at/research/bon-kyoung-koo/
 Total cost 195˙454 €
 EC max contribution 195˙454 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2014
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2016
 Duration (year-month-day) from 2016-01-01   to  2018-11-01

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE UK (CAMBRIDGE) coordinator 195˙454.00

Map

 Project objective

The epithelium of small intestine has a higher self-renewal rate than any other mammalian tissues. This is fuelled by the intestinal stem cells which reside near the bottom of crypt. Tight regulation of the adult stem cell number is required to sustain an adequately sized population for optimal tissue homeostasis. Mis-regulation of homeostasis results in either hyperplasia or loss of stem cells. Although transcriptional regulation of stem cell homeostasis regarding multipotency and differentiation has been extensively studied, only little is known about post-translational modifications. A key mechanism of post-translational modification is ubiquitination by the ubiquitin system (US).

The in vitro primary intestinal organoid culture system recapitulates in vivo epithelium organization, is amenable to essentially all experimental technologies that have been developed for cell lines, and therefore serves as a novel, robust and powerful model system. Taking advantage of the intestinal organoids, this project is aimed to investigate the role of US on the homeostatic control of stem cell maintenance and differentiation in crypt stem cells by: 1. Screening of US required for stem cell number regulation by CRISPR/Cas9 technology; 2. Characterization of the expression pattern and sub-cellular localization of candidate US; 3. Examining the effect of candidate US in signalling pathways involved in the homeostatic control by reporter assay and target gene expression; 4. Identifying target proteins of candidate US using surface proteome analysis, IP-mass, BioID or in vitro ubiquitination of arrayed proteins; 5. Performing biochemical and cell biological analysis of candidate US with its identified target proteins and signalling pathway components in vitro; 6. Generation of conditional knockout mouse models to elucidate the function of candidate US in vivo; 7. Conducting a small molecule screening for inhibitors that attenuate hyperplasia phenotype caused by E3 ligases mutation.

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The information about "INTESTINEUB" are provided by the European Opendata Portal: CORDIS opendata.

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