Opendata, web and dolomites

IntestineUb

The role of Ubiquitin System on the homeostatic control of stem cell maintenance and differentiation in crypt stem cells

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 IntestineUb project word cloud

Explore the words cloud of the IntestineUb project. It provides you a very rough idea of what is the project "IntestineUb" about.

maintenance    transcriptional    e3    elucidate    localization    effect    reporter    epithelium    adequately    advantage    self    organoids    inhibitors    signalling    performing    conditional    tissue    mutation    knockout    model    ubiquitin    phenotype    vitro    characterization    assay    homeostasis    population    essentially    components    candidate    tight    serves    attenuate    lines    screening    function    organoid    fuelled    mouse    caused    arrayed    mis    small    adult    primary    post    cells    ubiquitination    culture    proteins    regarding    cellular    technologies    differentiation    powerful    expression    conducting    renewal    modifications    sub    rate    experimental    extensively    either    reside    stem    proteome    cell    tissues    pattern    gene    little    biochemical    generation    crypt    mammalian    homeostatic    multipotency    vivo    crispr    sized    ligases    ip    intestinal    amenable    optimal    hyperplasia    cas9    modification    regulation    mass    organization    intestine    mechanism    sustain    models    bioid    surface    translational    molecule    near    biological    recapitulates   

Project "IntestineUb" data sheet

The following table provides information about the project.

Coordinator
THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE 

Organization address
address: TRINITY LANE THE OLD SCHOOLS
city: CAMBRIDGE
postcode: CB2 1TN
website: www.cam.ac.uk

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Project website https://www.imba.oeaw.ac.at/research/bon-kyoung-koo/
 Total cost 195˙454 €
 EC max contribution 195˙454 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2014
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2016
 Duration (year-month-day) from 2016-01-01   to  2018-11-01

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE UK (CAMBRIDGE) coordinator 195˙454.00

Map

 Project objective

The epithelium of small intestine has a higher self-renewal rate than any other mammalian tissues. This is fuelled by the intestinal stem cells which reside near the bottom of crypt. Tight regulation of the adult stem cell number is required to sustain an adequately sized population for optimal tissue homeostasis. Mis-regulation of homeostasis results in either hyperplasia or loss of stem cells. Although transcriptional regulation of stem cell homeostasis regarding multipotency and differentiation has been extensively studied, only little is known about post-translational modifications. A key mechanism of post-translational modification is ubiquitination by the ubiquitin system (US).

The in vitro primary intestinal organoid culture system recapitulates in vivo epithelium organization, is amenable to essentially all experimental technologies that have been developed for cell lines, and therefore serves as a novel, robust and powerful model system. Taking advantage of the intestinal organoids, this project is aimed to investigate the role of US on the homeostatic control of stem cell maintenance and differentiation in crypt stem cells by: 1. Screening of US required for stem cell number regulation by CRISPR/Cas9 technology; 2. Characterization of the expression pattern and sub-cellular localization of candidate US; 3. Examining the effect of candidate US in signalling pathways involved in the homeostatic control by reporter assay and target gene expression; 4. Identifying target proteins of candidate US using surface proteome analysis, IP-mass, BioID or in vitro ubiquitination of arrayed proteins; 5. Performing biochemical and cell biological analysis of candidate US with its identified target proteins and signalling pathway components in vitro; 6. Generation of conditional knockout mouse models to elucidate the function of candidate US in vivo; 7. Conducting a small molecule screening for inhibitors that attenuate hyperplasia phenotype caused by E3 ligases mutation.

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "INTESTINEUB" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "INTESTINEUB" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.3.2.)

COMLIB (2019)

Communists into Liberals: The Transformation and Demise of the Left as Precursor to the Illiberal Turn in Poland

Read More  

secretPOL (2019)

The PIDE and Portuguese Society under the Salazar Dictatorship 1945-1974: Fear, Self-Policing, Accommodation.

Read More  

FreeDigital (2019)

The impact of 'free' digital offers on individual behavior and its implications for consumer and data protection laws

Read More