Explore the words cloud of the HERPES project. It provides you a very rough idea of what is the project "HERPES" about.
The following table provides information about the project.
|Coordinator Country||Germany [DE]|
|Total cost||1˙994˙375 €|
|EC max contribution||1˙994˙375 € (100%)|
1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
|Duration (year-month-day)||from 2017-05-01 to 2022-04-30|
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|1||JULIUS-MAXIMILIANS-UNIVERSITAT WURZBURG||DE (WUERZBURG)||coordinator||1˙994˙375.00|
Herpes simplex virus 1 (HSV-1) is an important human pathogen, which intensively interacts with the cellular transcriptional machinery at multiple levels during lytic infection. Employing next-generation sequencing to study RNA synthesis, processing and translation in short intervals throughout lytic HSV-1 infection, my laboratory made the surprising observation that HSV-1 triggers widespread disruption of transcription termination of cellular but not viral genes. Transcription commonly extends for tens-of-thousands of nucleotides beyond poly(A)-sites and into downstream genes. In contrast to textbook knowledge, HSV-1 infection does not inhibit splicing but induces a broad range of aberrant splicing events associated with disruption of transcription termination. Exploring these fascinating phenomena will provide fundamental insights into RNA biology of human cells. The proposed work combines both hypothesis-driven and innovative unbiased screening approaches. I will utilise cutting-edge methodology ranging from high-throughput studies to advanced single molecule imaging. Thereby, I will detail the molecular mechanisms responsible for disruption of transcription termination and aberrant splicing. I will identify novel cellular proteins governing transcription termination using a genome-wide Cas9-knockout screen. I will develop RNA aptamer technology to visualise and track single RNA molecules suffering from poly(A) read-through. I will elucidate why transcription termination of some cellular and all viral genes remains unaltered throughout infection. I hypothesize that the alterations in RNA processing are depicted by specific changes in RNA Polymerase II CTD phosphorylation and in the associated proteins. I will characterise these dynamic changes using mNET-seq and quantitative proteomics. Finally, data-driven quantitative bioinformatic modelling will detail how the coupling of RNA synthesis, processing, export, stability and translation is orchestrated by HSV-1.
|year||authors and title||journal||last update|
Florian Erhard, Marisa A. P. Baptista, Tobias Krammer, Thomas Hennig, Marius Lange, Panagiota Arampatzi, Christopher S. JÃ¼rges, Fabian J. Theis, Antoine-Emmanuel Saliba, Lars DÃ¶lken
scSLAM-seq reveals core features of transcription dynamics in single cells
published pages: 419-423, ISSN: 0028-0836, DOI: 10.1038/s41586-019-1369-y
Florian Erhard, Anne Halenius, Cosima Zimmermann, Anne L\'Hernault, Daniel J Kowalewski, Michael P Weekes, Stefan Stevanovic, Ralf Zimmer, Lars DÃ¶lken
Improved Ribo-seq enables identification of cryptic translation events
published pages: 363-366, ISSN: 1548-7091, DOI: 10.1038/nmeth.4631
|Nature Methods 15/5||2019-04-18|
Marisa A P Baptista, Lars DÃ¶lken
RNA dynamics revealed by metabolic RNA labeling and biochemical nucleoside conversions
published pages: 171-172, ISSN: 1548-7091, DOI: 10.1038/nmeth.4608
|Nature Methods 15/3||2019-04-18|
Emanuel Wyler, Jennifer Menegatti, Vedran Franke, Christine Kocks, Anastasiya Boltengagen, Thomas Hennig, Kathrin Theil, Andrzej Rutkowski, Carmelo Ferrai, Laura Baer, Lisa Kermas, Caroline Friedel, Nikolaus Rajewsky, Altuna Akalin, Lars DÃ¶lken, Friedrich GrÃ¤sser, Markus Landthaler
Widespread activation of antisense transcription of the host genome during herpes simplex virus 1 infection
published pages: , ISSN: 1474-760X, DOI: 10.1186/s13059-017-1329-5
|Genome Biology 18/1||2019-04-18|
Thomas Hennig, Marco Michalski, Andrzej J. Rutkowski, Lara Djakovic, Adam W. Whisnant, Marie-Sophie Friedl, Bhaskar Anand Jha, Marisa A. P. Baptista, Anne Lâ€™Hernault, Florian Erhard, Lars DÃ¶lken, Caroline C. Friedel
HSV-1-induced disruption of transcription termination resembles a cellular stress response but selectively increases chromatin accessibility downstream of genes
published pages: e1006954, ISSN: 1553-7374, DOI: 10.1371/journal.ppat.1006954
|PLOS Pathogens 14/3||2019-04-18|
Christopher JÃ¼rges, Lars DÃ¶lken, Florian Erhard
Dissecting newly transcribed and old RNA using GRAND-SLAM
published pages: i218-i226, ISSN: 1367-4803, DOI: 10.1093/bioinformatics/bty256
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