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MitoQuant SIGNED

Development of Deep-UV Quantitative Microscopy for the Study of Mitochondrial Dysfunction

Total Cost €

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EC-Contrib. €

0

Partnership

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 MitoQuant project word cloud

Explore the words cloud of the MitoQuant project. It provides you a very rough idea of what is the project "MitoQuant" about.

matching    apertures    little    reconstruction    trained    noising    interplay    machine    quantitative    compiled    mitochondria    start    microscopy    deep    good    dna    image    overexpression    editor    numerical    fluorescently    techniques    molecular    network    play    science    counter    signal    dysfunction    material    adds    microscope    instrument    algorithms    track    wavelengths    vital    experiments    reduces    technique    labelled    overshadowing    dynamics    contextual    originally    issue    record    deductions    continued    circumvents    hence    free    quality    establishing    linked    diabetes    illumination    signals    resolution    neural    routines    proteins    microscopes    optics    sparse    light    label    excellent    gene    cell    suited    levels    sequences    modifies    concurrently    diseases    strives    turn    skews    possibility    time    machinery    surprising    presented    simultaneously    organelles    worry    mitochondrial    extract    crispr    uv    de    neurodegeneration    highest    cas9    classify    live    autofluorescence    fluorescence    first    specificity    researcher    100nm    contrast    superresolution    imaging    fluorescent    learning    transfection    mitoquant    cellular    employ    structured    building   

Project "MitoQuant" data sheet

The following table provides information about the project.

Coordinator
UNIVERSITETET I TROMSOE - NORGES ARKTISKE UNIVERSITET 

Organization address
address: HANSINE HANSENS VEG 14
city: TROMSO
postcode: 9019
website: http://uit.no/

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Norway [NO]
 Total cost 202˙158 €
 EC max contribution 202˙158 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2018
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2019
 Duration (year-month-day) from 2019-07-01   to  2021-06-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNIVERSITETET I TROMSOE - NORGES ARKTISKE UNIVERSITET NO (TROMSO) coordinator 202˙158.00

Map

 Project objective

Mitochondria play a vital role in the cellular machinery, hence it is little surprising that their dysfunction has been linked to many diseases, from diabetes to neurodegeneration. However, as many studies on the interplay of organelles and molecular dynamics often employ fluorescence microscopy, a continued worry overshadowing findings and deductions is the possibility that the transfection-induced overexpression of fluorescent proteins skews the obtained results. A recent approach, the gene editor CRISPR-CAS9, which modifies rather than adds DNA sequences, circumvents this issue, but in turn often reduces the available signal levels. To counter low signals and yet offer highest resolution and specificity, MitoQuant aims to image contextual mitochondrial information with label-free superresolution, while simultaneously enhance image quality of specific but sparse fluorescently labelled proteins of interest through recently presented de-noising routines based on machine learning. Therefore, the development of a novel instrument to provide adequate resolution and contrast, matching label-based live-cell superresolution techniques like structured illumination microscopy, is the first main goal of this project. The proposed microscope will work in the deep UV range and employ dedicated optics originally developed for material science to provide high numerical apertures at short wavelengths, thus enabling live-cell imaging in the 100nm range. Concurrently, a neural network will be compiled and trained to enhance signals under low-light conditions and to extract and classify cellular organelles based on their quantitative phase and autofluorescence information. Building on an excellent track record of developing application-tailored microscopes as well as advanced image reconstruction and processing algorithms particularly suited for live-cell superresolution, the researcher strives to start with first live-cell experiments in good time after establishing the technique.

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The information about "MITOQUANT" are provided by the European Opendata Portal: CORDIS opendata.

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