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MitoQuant SIGNED

Development of Deep-UV Quantitative Microscopy for the Study of Mitochondrial Dysfunction

Total Cost €

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EC-Contrib. €

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Partnership

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 MitoQuant project word cloud

Explore the words cloud of the MitoQuant project. It provides you a very rough idea of what is the project "MitoQuant" about.

machine    specificity    experiments    instrument    live    uv    molecular    illumination    gene    structured    algorithms    reconstruction    free    skews    trained    start    neurodegeneration    mitochondria    fluorescence    learning    contextual    100nm    issue    deep    network    levels    little    optics    noising    label    interplay    play    image    wavelengths    apertures    quantitative    superresolution    possibility    hence    neural    counter    suited    numerical    employ    dysfunction    vital    de    simultaneously    contrast    imaging    microscope    linked    fluorescently    extract    mitochondrial    editor    sequences    surprising    record    resolution    compiled    strives    time    microscopes    transfection    cellular    dynamics    adds    classify    circumvents    microscopy    highest    quality    continued    deductions    machinery    signals    mitoquant    crispr    labelled    signal    modifies    routines    overexpression    dna    reduces    technique    sparse    diseases    fluorescent    diabetes    autofluorescence    science    concurrently    techniques    building    originally    material    establishing    good    overshadowing    track    cas9    matching    presented    turn    worry    cell    researcher    first    proteins    organelles    light    excellent   

Project "MitoQuant" data sheet

The following table provides information about the project.

Coordinator
UNIVERSITETET I TROMSOE - NORGES ARKTISKE UNIVERSITET 

Organization address
address: HANSINE HANSENS VEG 14
city: TROMSO
postcode: 9019
website: http://uit.no/

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Norway [NO]
 Total cost 202˙158 €
 EC max contribution 202˙158 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2018
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2019
 Duration (year-month-day) from 2019-07-01   to  2021-06-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNIVERSITETET I TROMSOE - NORGES ARKTISKE UNIVERSITET NO (TROMSO) coordinator 202˙158.00

Map

 Project objective

Mitochondria play a vital role in the cellular machinery, hence it is little surprising that their dysfunction has been linked to many diseases, from diabetes to neurodegeneration. However, as many studies on the interplay of organelles and molecular dynamics often employ fluorescence microscopy, a continued worry overshadowing findings and deductions is the possibility that the transfection-induced overexpression of fluorescent proteins skews the obtained results. A recent approach, the gene editor CRISPR-CAS9, which modifies rather than adds DNA sequences, circumvents this issue, but in turn often reduces the available signal levels. To counter low signals and yet offer highest resolution and specificity, MitoQuant aims to image contextual mitochondrial information with label-free superresolution, while simultaneously enhance image quality of specific but sparse fluorescently labelled proteins of interest through recently presented de-noising routines based on machine learning. Therefore, the development of a novel instrument to provide adequate resolution and contrast, matching label-based live-cell superresolution techniques like structured illumination microscopy, is the first main goal of this project. The proposed microscope will work in the deep UV range and employ dedicated optics originally developed for material science to provide high numerical apertures at short wavelengths, thus enabling live-cell imaging in the 100nm range. Concurrently, a neural network will be compiled and trained to enhance signals under low-light conditions and to extract and classify cellular organelles based on their quantitative phase and autofluorescence information. Building on an excellent track record of developing application-tailored microscopes as well as advanced image reconstruction and processing algorithms particularly suited for live-cell superresolution, the researcher strives to start with first live-cell experiments in good time after establishing the technique.

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The information about "MITOQUANT" are provided by the European Opendata Portal: CORDIS opendata.

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